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Protocol
The Isolation of DNA Sequences Flanking Tn5 Transposon Insertions by Inverse PCR
Because of its versatility, the Tn5 transposon has become a powerful tool in the classical genetic studies of Gram-negative bacteria. The Tn5 transposon is functional in a broad range of Gram-negative bacteria...
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Article
Purification, characterization and gene sequence analysis of a novel cytochrome c co-induced with reductive dechlorination activity in Desulfomonile tiedjei DCB-1
The sulfate-reducing bacterium, Desulfomonile tiedjei DCB-1, conserves energy for growth from reductive dechlorination of 3-chlorobenzoate via halorespiration. To understand this respiratory process better, we ex...
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Article
Evidence for chemiosmotic coupling of reductive dechlorination and ATP synthesis in Desulfomonile tiedjei
Desulfomonile tiedjei (strain DCB-1) was previously shown to conserve energy for growth from reductive dechlorination of 3-chlorobenzoate coupled to formate oxidation. We tested the hypothesis that a chemiosmoti...
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Article
Strain DCB-1 conserves energy for growth from reductive dechlorination coupled to formate oxidation
Strain DCB-1 is a strict anaerobe capable of the reductive dechlorination of chlorobenzoates. The effect of dechlorination on the yield of pure cultures of DCB-1 was tested. Cultures were incubated with format...