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  1. No Access

    Article

    Heritable DNA-free genome editing of canola (Brassica napus L.) using PEG-mediated transfection of isolated protoplasts

    Application of new genome editing technologies for plant improvement depends on efficient delivery of editing components into plant cells. For editing of canola, which is an important oil crop, CRISPR/Cas9 and Ag...

    Vladimir Sidorov, Dafu Wang, Ervin D. Nagy… in In Vitro Cellular & Developmental Biology … (2022)

  2. No Access

    Protocol

    Agrobacterium-Mediated Maize Transformation: Immature Embryos Versus Callus

    Transformation with Agrobacterium tumefaciens is the preferred method for delivery of transgenes into a wide range of plant species including maize. Optimized protocols for the Agrobacterium-mediated transformati...

    Vladimir Sidorov, David Duncan in Transgenic Maize (2009)

  3. No Access

    Article

    Complementation in somatic hybrids indicates four types of nitrate reductase deficient lines in Nicotiana plumbaginifolia

    Allelism of nine nitrate reductase deficient (NR−) Nicotiana plumbaginifolia cell lines was tested by complementation after protoplast fusion. Complementation was recognized by the appearance of somatic hybrid co...

    László Márton, Vladimir Sidorov, Grazia Biasini… in Molecular and General Genetics MGG (1982)