20 Result(s)
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Valentin Borshchevskiy
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Article
Bright and stable monomeric green fluorescent protein derived from StayGold
The high brightness and photostability of the green fluorescent protein StayGold make it a particularly attractive probe for long-term live-cell imaging; however, its dimeric nature precludes its application a...
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Article
Mechanisms of inward transmembrane proton translocation
Proton transport is indispensable for cell life. It is believed that molecular mechanisms of proton movement through different types of proton-conducting molecules have general universal features. However, elu...
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Open AccessSub-millisecond conformational dynamics of the A2A adenosine receptor revealed by single-molecule FRET
The complex pharmacology of G-protein-coupled receptors (GPCRs) is defined by their multi-state conformational dynamics. Single-molecule Förster Resonance Energy Transfer (smFRET) is well suited to quantify dy...
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Protocol
Development and Characterization of Flavin-Binding Fluorescent Proteins, Part I: Basic Characterization
Flavin-based fluorescent proteins (FbFPs) are small fluorescent proteins derived from light-oxygen-voltage (LOV) domains. The proteins bind ubiquitous endogenous flavins as chromophores and can be used as vers...
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Article
Open AccessStructural basis for receptor selectivity and inverse agonism in S1P5 receptors
The bioactive lysophospholipid sphingosine-1-phosphate (S1P) acts via five different subtypes of S1P receptors (S1PRs) - S1P1-5. S1P5 is predominantly expressed in nervous and immune systems, regulating the egres...
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Article
True-atomic-resolution insights into the structure and functional role of linear chains and low-barrier hydrogen bonds in proteins
Hydrogen bonds are fundamental to the structure and function of biological macromolecules and have been explored in detail. The chains of hydrogen bonds (CHBs) and low-barrier hydrogen bonds (LBHBs) were propo...
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Article
Open AccessHigh-pressure crystallography shows noble gas intervention into protein-lipid interaction and suggests a model for anaesthetic action
In this work we examine how small hydrophobic molecules such as inert gases interact with membrane proteins (MPs) at a molecular level. High pressure atmospheres of argon and krypton were used to produce noble...
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Protocol
Time-Resolved UV-VIS Spectroscopy of Microbial Rhodopsins
Absorption of light quanta by microbial rhodopsins (or more generally by retinal proteins) leads to conversion of the light energy to the generation of transmembrane anion or cation gradients, optically gated ...
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Article
Extreme dependence of Chloroflexus aggregans LOV domain thermo- and photostability on the bound flavin species
Light-oxygen-voltage (LOV) domains are common photosensory modules that found many applications in fluorescence microscopy and optogenetics. Here, we show that the Chloroflexus aggregans LOV domain can bind diffe...
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Article
Open AccessStructure-based insights into evolution of rhodopsins
Rhodopsins, most of which are proton pumps generating transmembrane electrochemical proton gradients, span all three domains of life, are abundant in the biosphere, and could play a crucial role in the early e...
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Article
Open AccessAuthor Correction: Small-wedge synchrotron and serial XFEL datasets for Cysteinyl leukotriene GPCRs
A Correction to this paper has been published: https://doi.org/10.1038/s41597-020-00759-w
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Article
Open AccessSmall-wedge synchrotron and serial XFEL datasets for Cysteinyl leukotriene GPCRs
Structural studies of challenging targets such as G protein-coupled receptors (GPCRs) have accelerated during the last several years due to the development of new approaches, including small-wedge and serial c...
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Article
Open AccessMolecular mechanism of light-driven sodium pum**
The light-driven sodium-pum** rhodopsin KR2 from Krokinobacter eikastus is the only non-proton cation active transporter with demonstrated potential for optogenetics. However, the existing structural data on KR...
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Article
Open AccessStructural basis of ligand selectivity and disease mutations in cysteinyl leukotriene receptors
Cysteinyl leukotriene G protein-coupled receptors CysLT1 and CysLT2 regulate pro-inflammatory responses associated with allergic disorders. While selective inhibition of CysLT1R has been used for treating asthma ...
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Article
Open AccessUnique structure and function of viral rhodopsins
Recently, two groups of rhodopsin genes were identified in large double-stranded DNA viruses. The structure and function of viral rhodopsins are unknown. We present functional characterization and high-resolut...
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Article
A thermostable flavin-based fluorescent protein from Chloroflexus aggregans: a framework for ultra-high resolution structural studies
Light-Oxygen-Voltage (LOV) domains are conserved parts of photoreceptors in plants, bacteria and fungi that bind flavins as chromophores and detect blue light. In the past, LOV domain variants have been develo...
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Article
Open AccessEfficient non-cytotoxic fluorescent staining of halophiles
Research on halophilic microorganisms is important due to their relation to fundamental questions of survival of living organisms in a hostile environment. Here we introduce a novel method to stain halophiles ...
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Article
Crystal structure of a light-driven sodium pump
Crystal structures of the microbial rhodopsin KR2, a recently discovered light-driven sodium pump, reveal the translocation pathway of sodium ions and shed light on the molecular mechanism of ion pum**.
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Article
X-ray structure of a CDP-alcohol phosphatidyltransferase membrane enzyme and insights into its catalytic mechanism
Phospholipids have major roles in the structure and function of all cell membranes. Most integral membrane proteins from the large CDP-alcohol phosphatidyltransferase family are involved in phospholipid biosyn...
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Article
Active arrestin proteins crystallized
Arrestin proteins regulate cellular signalling cascades initiated by ubiquitous G-protein-coupled receptors. Crystal structures reveal that two arrestins undergo similar structural changes on activation. See Lett...
