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Article
Rapid manufacturing of non-activated potent CAR T cells
Chimaeric antigen receptor (CAR) T cells can generate durable clinical responses in B-cell haematologic malignancies. The manufacturing of these T cells typically involves their activation, followed by viral t...
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Article
Heavy metal protease takes a tiki torch to HIV assembly
The plasma membrane–associated metalloprotease TRABD2A degrades the HIV Gag polyprotein and restricts virion assembly.
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Article
Open AccessLongitudinal HIV sequencing reveals reservoir expression leading to decay which is obscured by clonal expansion
After initiating antiretroviral therapy (ART), a rapid decline in HIV viral load is followed by a long period of undetectable viremia. Viral outgrowth assay suggests the reservoir continues to decline slowly. ...
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Article
Open AccessClinical use of lentiviral vectors
Viral vectors provide an efficient means for modification of eukaryotic cells, and their use is now commonplace in academic laboratories and industry for both research and clinical gene therapy applications. L...
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Article
Open AccessMeasuring integrated HIV DNA ex vivo and in vitro provides insights about how reservoirs are formed and maintained
The identification of the most appropriate marker to measure reservoir size has been a great challenge for the HIV field. Quantitative viral outgrowth assay (QVOA), the reference standard to quantify the amoun...
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Article
Open AccessBeyond the replication-competent HIV reservoir: transcription and translation-competent reservoirs
Recent years have seen a substantial increase in the number of tools available to monitor and study HIV reservoirs. Here, we discuss recent technological advances that enable an understanding of reservoir dyna...
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Article
Open AccessHIV latency and integration site placement in five cell-based models
HIV infection can be treated effectively with antiretroviral agents, but the persistence of a latent reservoir of integrated proviruses prevents eradication of HIV from infected individuals. The chromosomal en...
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Chapter
Tolerizing Mice to Human Leukocytes: A Step Toward the Production of Monoclonal Antibodies Specific for Human Dendritic Cells
Despite several attempts to isolate a mAb specific for human dendritic cells, none currently exists. Recent attempts have utilized an improved dendritic cell purification method to prepare immunogens1 and a rapid...
