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Article
Governance paradox: implications from Japan’s national parks for managing complex protected areas
Herein, we discuss the governance implications for emerging protected areas with complexity in the 2020s by analyzing public–private partnership frameworks in Japan’s national parks. First, we summarize previo...
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Article
Immediate and cumulative stresses associated with the multiscale impacts of ecotourism on ecological status and resilience
Anthropogenic impacts on ecosystems across spatiotemporal scales are expanding globally, undermining ecosystem resilience and increasing the risk of regime shifts within ecosystems. Governance incorporating so...
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Article
Open AccessEmpowering voluntary approaches for environmental sustainability and resilient communities: a case study from Okinawa, Japan
What can government do to secure environmental sustainability and resilient communities? We discuss an emerging policy approach in natural tourism resource management in the Okinawa Prefecture using the case o...
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Article
Open AccessEr:YAG laser-induced cavitation can activate irrigation for the removal of intraradicular biofilm
We investigated the biofilm removal effects of laser activated irrigation (LAI) using a pig model, focusing on the impact of the fiber tip position, and used a high-speed camera to observe the occurrence and p...
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Article
Open AccessAn experimental intraradicular biofilm model in the pig for evaluating irrigation techniques
We established an in vivo intraradicular biofilm model of apical periodontitis in pigs in which we compared the efficacy of different irrigant activation techniques for biofilm removal.
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Chapter
Governance for Protected Areas “Beyond the Boundary”—A Conceptual Framework for Biodiversity Conservation in the Anthropocene
While biodiversity enhances human resilience to the impacts of rapid global change, the capacity for protected areas to adequately contribute to biodiversity conservation is increasingly questioned. Convention...
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Article
Analysis of the Governance Structures in Japan’s Biosphere Reserves: Perspectives from Bottom–Up and Multilevel Characteristics
This paper analyzes the governance structures of Biosphere Reserves (BRs) in Japan by focusing on six criteria that elucidate the main characteristics therein: general information (nomination process, year of ...
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Chapter
Field Survey Key Informant Interviews in Sustainability Science: Costa Rica’s PES Policy of Changing Focus from Quantity to Quality
This paper attempts to elucidate the current challenges to the implementation of Costa Rica’s Payments for Environmental Services (PES) for agroforestry. By interviewing important stakeholders in program imple...
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Article
Public Perceptions of Nature and Landscape Preference in Singapore
Widespread urbanization has led to urban areas becoming increasingly seen as sites for biodiversity conservation. Urban and landscape planners are required to concurrently tackle environmental and social issue...
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Article
A549 and PLC/PRF/5 cells can support the efficient propagation of swine and wild boar hepatitis E virus (HEV) strains: demonstration of HEV infectivity of porcine liver sold as food
Recent evidence has indicated the cross-species transmission of hepatitis E virus (HEV) from pigs and wild boars to humans, causing zoonosis, mostly via consumption of uncooked or undercooked animal meat/visce...
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Article
Determination of the 5′-terminal sequence of subgenomic RNA of hepatitis E virus strains in cultured cells
Using RNA preparations extracted from PLC/PRF/5 cells transfected with infectious genotype 3 hepatitis E virus (HEV) cDNA clones or inoculated with a fecal suspension containing a genotype 4 HEV, the 5′-termin...
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Article
Modification of Monodisperse Colloidal Silica by Radical Copolymerizatoin of Cationic Surface Active Vinyl Monomers
Adsorption and polymer modification of colloidal silica by radical copolymerization of surface active monomers, i.e., polymerizable surfactant having a vinyl end group, were investigated. Radical copolymerization...
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Article
Monoclonal antibodies raised against the ORF3 protein of hepatitis E virus (HEV) can capture HEV particles in culture supernatant and serum but not those in feces
Ten murine monoclonal antibodies (MAbs) against a synthetic peptide corresponding to the well-conserved, C-terminal 24-amino acid portion of ORF3 protein of hepatitis E virus (HEV) were produced and characteri...
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Article
Production of monoclonal antibodies against hepatitis E virus capsid protein and evaluation of their neutralizing activity in a cell culture system
Nine murine monoclonal antibodies (mAbs) generated against a recombinant ORF2 protein (amino acids 111–660) of a genotype 4 hepatitis E virus (HEV) strain recognized four sets of epitopes by pairwise competiti...
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Article
Successful expression in pollen of various plant species of in vitro synthesized mRNA introduced by particle bombardment
Gold particles coated with β-glucuronidase (GUS) mRNA with a 5′ cap structure that had been synthesized in vitro were introduced, by use of a pneumatic particle gun, into pollen grains of lily (Lilium longiflorum
