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Article
Making genome editing a success story in Africa
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Article
Open AccessSingle-cell profiling of Anopheles gambiae spermatogenesis defines the onset of meiotic silencing and premeiotic overexpression of the X chromosome
Understanding development and genetic regulation in the Anopheles gambiae germline is essential to engineer effective genetic control strategies targeting this malaria mosquito vector. These include targeting the...
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Article
Open AccessGene-drive suppression of mosquito populations in large cages as a bridge between lab and field
CRISPR-based gene-drives targeting the gene doublesex in the malaria vector Anopheles gambiae effectively suppressed the reproductive capability of mosquito populations reared in small laboratory cages. To bridge...
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Article
Open AccessAuthor Correction: A male-biased sex-distorter gene drive for the human malaria vector Anopheles gambiae
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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Article
Open AccessA male-biased sex-distorter gene drive for the human malaria vector Anopheles gambiae
Only female insects transmit diseases such as malaria, dengue and Zika; therefore, control methods that bias the sex ratio of insect offspring have long been sought. Genetic elements such as sex-chromosome dri...
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Article
Open AccessTranscriptional variation of sensory-related genes in natural populations of Aedes albopictus
The Asian tiger mosquito, Aedes albopictus, is a highly dangerous invasive vector of numerous medically important arboviruses including dengue, chikungunya and Zika. In four decades it has spread from tropical So...
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Article
Open AccessHigh-resolution transcriptional profiling of Anopheles gambiae spermatogenesis reveals mechanisms of sex chromosome regulation
Although of high priority for the development of genetic tools to control malaria-transmitting mosquitoes, only a few germline-specific regulatory regions have been characterised to date and the presence of gl...
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Article
Open AccessPublisher Correction: Introgression of a synthetic sex ratio distortion system from Anopheles gambiae into Anopheles arabiensis
A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper.
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Article
Open AccessIntrogression of a synthetic sex ratio distortion system from Anopheles gambiae into Anopheles arabiensis
I-PpoI is a homing endonuclease that has a high cleavage activity and specificity for a conserved sequence within the ribosomal rDNA repeats, located in a single cluster on the Anopheles gambiae X chromosome. Thi...
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Article
Open AccessMolecular tools and genetic markers for the generation of transgenic sexing strains in Anopheline mosquitoes
Malaria is a serious global health burden, affecting more than 200 million people each year in over 90 countries, predominantly in Africa, Asia and the Americas. Since the year 2000, a concerted effort to comb...
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Article
Open AccessA CRISPR–Cas9 gene drive targeting doublesex causes complete population suppression in caged Anopheles gambiae mosquitoes
Complete population collapse of malaria vector Anopheles gambiae in cages is achieved using a gene drive that targets doublesex.
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Article
Open AccessDeciphering the olfactory repertoire of the tiger mosquito Aedes albopictus
The Asian tiger mosquito Aedes albopictus is a highly invasive species and competent vector of several arboviruses (e.g. dengue, chikungunya, Zika) and parasites (e.g. dirofilaria) of public health importance. Co...
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Article
Open AccessA CRISPR-Cas9 sex-ratio distortion system for genetic control
Genetic control aims to reduce the ability of insect pest populations to cause harm via the release of modified insects. One strategy is to bias the reproductive sex ratio towards males so that a population de...
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Article
A CRISPR-Cas9 gene drive system targeting female reproduction in the malaria mosquito vector Anopheles gambiae
Development of a CRISPR/Cas9-based gene drive system in Anopheles gambiae, the main vector for the malaria parasite, paves the way for control of this pest insect.
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Article
Open AccessThe germline of the malaria mosquito produces abundant miRNAs, endo-siRNAs, piRNAs and 29-nt small RNAs
Small RNAs include different classes essential for endogenous gene regulation and cellular defence against genomic parasites. However, a comprehensive analysis of the small RNA pathways in the germline of the ...
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Article
Open AccessDisruption of aminergic signalling reveals novel compounds with distinct inhibitory effects on mosquito reproduction, locomotor function and survival
Insecticide resistance amongst disease vectors is a growing problem and novel compounds are needed. Biogenic amines are important for neurotransmission and we have recently shown a potential role for these in ...
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Protocol
Mosquito Transgenic Technologies to Reduce Plasmodium Transmission
The ability to introduce genetic constructs of choice into the genome of Anopheles mosquitoes provides a valuable tool to study the molecular interactions between the Plasmodium parasite and its insect host. In t...
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Article
Open AccessA comprehensive gene expression atlas of sex- and tissue-specificity in the malaria vector, Anopheles gambiae
The mosquito, Anopheles gambiae, is the primary vector of human malaria, a disease responsible for millions of deaths each year. To improve strategies for controlling transmission of the causative parasite, Plasm...
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Article
Develo** transgenic Anopheles mosquitoes for the sterile insect technique
In the last 10 years the availability of the genome sequence of Anopheles gambiae and the development of a transgenic technology for several species of Anopheles mosquitoes have, in combination, helped in enablin...
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Article
Post-integration behavior of a Minos transposon in the malaria mosquito Anopheles stephensi
Transposable elements represent important tools to perform functional studies in insects. In Drosophila melanogaster, the remobilization properties of transposable elements have been utilized for enhancer-trappin...
