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Article
Absent daddy, but important father
Mixing maternal and paternal genomes is the base of plant sexual reproduction, but some so-called ‘haploid inducer lines’ lead to the formation of seeds bearing well-developed embryos with solely the maternal ...
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Protocol
Overview of In Vitro and In Vivo Doubled Haploid Technologies
Doubled haploids (DH) have become a powerful tool to assist in different basic research studies, and also in applied research. The principal (but not the only) and routine use of DH by breeding companies is to...
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Protocol
Maize In Planta Haploid Inducer Lines: A Cornerstone for Doubled Haploid Technology
Doubled haploid (DH) technology produces strictly homozygous fertile plant thanks to doubling the chromosomes of a haploid embryo/seedling. Haploid embryos are derived from either male or female germ line cell...
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Article
Puzzling out plant reproduction by haploid induction for innovations in plant breeding
Mixing maternal and paternal genomes in embryos is not only responsible for the evolutionary success of sexual reproduction, but is also a cornerstone of plant breeding. However, once an interesting gene combi...
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Article
Single and multiple gene knockouts by CRISPR–Cas9 in maize
The analysis of 93 mutant alleles in 18 genes demonstrated that CRISPR–Cas9 is a robust tool for targeted mutagenesis in maize, permitting efficient generation of single and multiple knockouts.
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Article
Open AccessFast virtual histology using X-ray in-line phase tomography: application to the 3D anatomy of maize develo** seeds
Despite increasing demand, imaging the internal structure of plant organs or tissues without the use of transgenic lines expressing fluorescent proteins remains a challenge. Techniques such as magnetic resonan...
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Article
Open AccessA deep transcriptomic analysis of pod development in the vanilla orchid (Vanilla planifolia)
Pods of the vanilla orchid (Vanilla planifolia) accumulate large amounts of the flavor compound vanillin (3-methoxy, 4-hydroxy-benzaldehyde) as a glucoside during the later stages of their development. At earlier...
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Article
Functional characterization of two new members of the caffeoyl CoA O-methyltransferase-like gene family from Vanilla planifolia reveals a new class of plastid-localized O-methyltransferases
Caffeoyl CoA O-methyltransferases (OMTs) have been characterized from numerous plant species and have been demonstrated to be involved in lignin biosynthesis. Higher plant species are known to have additional caf...
