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Article
Open AccessCommunity and single cell analyses reveal complex predatory interactions between bacteria in high diversity systems
A fundamental question in community ecology is the role of predator–prey interactions in food-web stability and species coexistence. Although microbial microcosms offer powerful systems to investigate it, inte...
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Article
Bacterial mock communities as standards for reproducible cytometric microbiome analysis
Flow cytometry has recently established itself as a tool to track short-term dynamics in microbial community assembly and link those dynamics with ecological parameters. However, instrumental configurations of...
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Article
Open AccessGastric bypass surgery in a rat model alters the community structure and functional composition of the intestinal microbiota independently of weight loss
Roux-en-Y gastric bypass (RYGB) surgery is a last-resort treatment to induce substantial and sustained weight loss in cases of severe obesity. This anatomical rearrangement affects the intestinal microbiota, b...
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Article
Open AccessFlow cytometric quantification, sorting and sequencing of methanogenic archaea based on F420 autofluorescence
The widely established production of CH4 from renewable biomass in industrial scale anaerobic reactors may play a major role in the future energy supply. It relies on methanogenic archaea as key organisms which r...
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Article
Open AccessCopy number variability of expression plasmids determined by cell sorting and Droplet Digital PCR
Plasmids are widely used for molecular cloning or production of proteins in laboratory and industrial settings. Constant modification has brought forth countless plasmid vectors whose characteristics in terms ...
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Article
Open AccessMycelia as a focal point for horizontal gene transfer among soil bacteria
Horizontal gene transfer (HGT) is a main mechanism of bacterial evolution endowing bacteria with new genetic traits. The transfer of mobile genetic elements such as plasmids (conjugation) requires the close pr...
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Article
Cytometric fingerprinting for analyzing microbial intracommunity structure variation and identifying subcommunity function
Functions of complex natural microbial communities are realized by single cells that contribute differently to the overall performance of a community. Usually, molecular and, more recently, deep-sequencing tec...
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Article
Charakterisierung von mikrobiellen Gemeinschaften
Cytomics and Proteomics can be used in combination to analyze structure, dynamics and function of microbial communities quantitatively and with high resolution. In order to understand the function of individua...
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Chapter
Resolution of Natural Microbial Community Dynamics by Community Fingerprinting, Flow Cytometry, and Trend Interpretation Analysis
Natural microbial communities generally have an unknown structure and composition because of their still not yet cultivable members. Therefore, understanding the relationships among the bacterial members, pred...
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Article
Advanced tool for characterization of microbial cultures by combining cytomics and proteomics
Flow cytometry approaches are applicable to recover sub-populations of microbial cultures in a purified form. To examine the characteristics of each sorted cell population, Omics technologies can be used for comp...
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Chapter
Characterization of A Synechocystis Phytochrome
Phytochromes are photoreceptors common to lower and higher plants. Plant phytochrome apoproteins autocatalytically attach phytochromobilin, a linear tetrapyrrole chromophore [1]. They regulate a wide variety o...
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Article
Characterisation of transcript initiation sites in ribosome-deficient barley plastids
Transcription of plastid genes in higher plants is driven by two RNA polymerases. One is encoded in the chloroplast, the other is encoded in the nucleus. RNA synthesis in ribosome-deficient plastids is perform...
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Article
Impaired splicing of the rps 12 transcript in ribosome-deficient plastids
Analysis of RNA maturation in ribosome-deficient plastids of four non-allelic barley mutants revealed an increased accumulation and altered processing of transcripts of the ribosomal protein gene CS12 (rps12) com...
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Article
Transcription and in vivo expression of aMicrocystis aeruginosa plasmid
A restriction map of the plasmid pMA1 ofMicrocystis aeruginosa is presented. The total RNA of the plasmid-containing strain HUB 5-2-4 was isolated and hybridized with the resident plasmid, pMA1, of the strain HUB...
