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Article
Open AccessProteome-wide prediction of bacterial carbohydrate-binding proteins as a tool for understanding commensal and pathogen colonisation of the vaginal microbiome
Bacteria use carbohydrate-binding proteins (CBPs), such as lectins and carbohydrate-binding modules (CBMs), to anchor to specific sugars on host surfaces. CBPs in the gut microbiome are well studied, but their...
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Article
Open AccessSingle human B cell-derived monoclonal anti-Candida antibodies enhance phagocytosis and protect against disseminated candidiasis
The high global burden of over one million annual lethal fungal infections reflects a lack of protective vaccines, late diagnosis and inadequate chemotherapy. Here, we have generated a unique set of fully huma...
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Article
Open AccessAssignment by Negative-Ion Electrospray Tandem Mass Spectrometry of the Tetrasaccharide Backbones of Monosialylated Glycans Released from Bovine Brain Gangliosides
Gangliosides, as plasma membrane-associated sialylated glycolipids, are antigenic structures and they serve as ligands for adhesion proteins of pathogens, for toxins of bacteria, and for endogenous proteins of...
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Article
Recognition of DHN-melanin by a C-type lectin receptor is required for immunity to Aspergillus
The C-type lectin receptor MelLec recognizes DHN-melanin in conidial spores of Aspergillus fumigatus and other DHN-melanized fungi, revealing an important role for this receptor in antifungal immunity in both mic...
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Article
Open AccessBinding of CLL Subset 4 B Cell Receptor Immunoglobulins to Viable Human Memory B Lymphocytes Requires a Distinctive IGKV Somatic Mutation
Amino acid replacement mutations in certain chronic lymphocytic leukemia (CLL) stereotyped B cell receptor (BCR) immunoglobulins (IGs) at defined positions within antigen-binding sites strongly imply antigen s...
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Article
Notum deacylates Wnt proteins to suppress signalling activity
Signalling by Wnt proteins is finely balanced to ensure normal development and tissue homeostasis while avoiding diseases such as cancer. This is achieved in part by Notum, a highly conserved secreted feedback...
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Reference Work Entry In depth
Neoglycolipid (NGL)-Based Glycan Microarray System for Ligand Discovery
It is now appreciated that carbohydrate–protein interactions are integral to many physiological processes and are directly or indirectly involved in the majority of disease processes, infective or noninfective...
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Living Reference Work Entry In depth
Neoglycolipid (NGL)-Based Glycan Microarray System for Ligand Discovery
It is now appreciated that carbohydrate–protein interactions are integral to many physiological processes and are directly or indirectly involved in the majority of disease processes, infective or noninfective...
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Article
Retraction Note: Oligosaccharide ligands for NKR-P1 protein activate NK cells and cytotoxicity
We wish to retract this Article owing to an inability to reproduce the results. This retraction has not been signed by K.B. and A.F., and M.P. is deceased (J.O’B. cannot be traced).
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Article
Supersite of immune vulnerability on the glycosylated face of HIV-1 envelope glycoprotein gp120
Some broadly neutralizing antibodies to HIV-1 recognize glycan-dependent epitopes on gp120. Now X-ray crystallography and EM approaches, along with functional analyses, reveal how one particular antibody (PGT1...
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Protocol
Neoglycolipid-Based Oligosaccharide Microarray System: Preparation of NGLs and Their Noncovalent Immobilization on Nitrocellulose-Coated Glass Slides for Microarray Analyses
Carbohydrate microarrays, since their advent in 2002, are revolutionizing studies of the molecular basis of protein–carbohydrate interactions both in endogenous recognition systems and pathogen–host interactio...
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Protocol
Neoglycolipid-Based “Designer” Oligosaccharide Microarrays to Define β-Glucan Ligands for Dectin-1
In this chapter, we describe the key steps of the “designer” oligosaccharide microarray approach we followed to prove the carbohydrate binding activity and define the oligosaccharide ligands for Dectin-1, an a...
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Article
Correction: Corrigendum: Receptor-binding specificity of pandemic influenza A (H1N1) 2009 virus determined by carbohydrate microarray
Nat. Biotechnol. 27, 797–799 (2009); published online 9 September 2009; corrected after print 5 February 2010 In the version of this article initially published, two acknowledgments were inadvertently omitted:...
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Article
GM1 structure determines SV40-induced membrane invagination and infection
It is unclear whether viral particles can induce membrane curvature. Binding of Simian virus 40 (SV40) to the GM1 ganglioside on host plasma membranes leads to membrane curvature and the formation of invaginat...
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Article
Receptor-binding specificity of pandemic influenza A (H1N1) 2009 virus determined by carbohydrate microarray
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Reference Work Entry In depth
Microarrays – A Key Technology for Glycobiology
Carbohydrate chains of , , and proteoglycans can mediate processes of biological and medical importance through their interactions with complementary proteins. The unraveling of these interactions is a prior...
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Article
Correction: Corrigendum: Engineering sweet targets for magic bullets
Nat. Biotechnol. 22, 1244–1245 (2004) On Page 1245, col. 2, paragraph 2, last line, “others3” should have been “others2”.
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Article
Engineering sweet targets for magic bullets
The cellular biosynthetic machinery can be harnessed to incorporate traceable markers into cell-surface glycans in vivo.
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Article
Oligosaccharide microarrays to decipher the glyco code
The oligosaccharide moieties of glycoproteins, glycolipids, proteoglycans and polysaccharides are highly diverse, the reason for this diversity is not yet understood. Neoglycolipid technology allows the genera...
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Article
Oligosaccharide microarrays for high-throughput detection and specificity assignments of carbohydrate-protein interactions
We describe microarrays of oligosaccharides as neoglycolipids and their robust display on nitrocellulose. The arrays are sourced from glycoproteins, glycolipids, proteoglycans, polysaccharides, whole organs, o...
