6 Result(s)
within
T. Tokiwa
Life Sciences
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Chapter
Application of Hepatocytes Isolated from Slaughtered Pig for Bioartificial Liver Support System
In this study, porcine primary hepatocytes were cultured with or without ascorbic acid 2-phosphate (Asc2-P) to compare the functional activities with rat primary hepatocytes. Ammonium metabolic activity and al...
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Chapter
Evaluation of a Fixed-Bed Type Bioreactor System Using Recombinant CHO Cells Producing Human Transferrin
To improve production efficiency of recombinant animal proteins, we evaluated a fixed bed type bioreactor, which we call circulatory flow bioreactor, using human transferrin-producing recombinant CHO cells. In...
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Article
Isolation and primary culture of adult pig hepatocytes
Hepatocytes were directly isolated from pig livers obtained from the abattoir and cultivated on a type I collagen-coated polystyrene surface. Digestion of the partial liver lobe resulted in an average yield of...
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Chapter
Morphology and Function of Rat Hepatocytes Cultured on Expanded Polytetrafluoroethylene Surface
for the development of a bioreactor using hepatocytes, particularly for a hybrid-type artificial liver, we study culture substrates in this paper with particular attention to a porous material, expanded polyte...
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Article
A method for transforming human liver epithelial cells by transfection using a plasmid containing SV40 early region gene
A method is described for establishing continuous cell lines of liver epithelial cells by transformation of cultured hepatocytes isolated by collagenase/dispase of adult human liver tissue. Between 2 to 5×105 hep...
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Chapter
Asbestos-Associated Chromosomal Changes in Human Mesothelial Cells
Epidemiological studies have established that exposure to asbestos fibers is the primary cause of mesothelioma in the industrialized world (Kannerstein and Churg 1980; Craighead and Mossman 1982). Because the ...
