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Article
Fracture Characterization and Rock Mass Damage Induced by Different Excavation Methods in the Horonobe URL of Japan
We conducted detailed fracture map** of the soft sedimentary rocks (uniaxial compressive strength of 10–20 MPa) in shaft walls at the Horonobe Underground Research Laboratory to characterize fractures and to...
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Chapter
Application of Hepatocytes Isolated from Slaughtered Pig for Bioartificial Liver Support System
In this study, porcine primary hepatocytes were cultured with or without ascorbic acid 2-phosphate (Asc2-P) to compare the functional activities with rat primary hepatocytes. Ammonium metabolic activity and al...
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Chapter
Evaluation of a Fixed-Bed Type Bioreactor System Using Recombinant CHO Cells Producing Human Transferrin
To improve production efficiency of recombinant animal proteins, we evaluated a fixed bed type bioreactor, which we call circulatory flow bioreactor, using human transferrin-producing recombinant CHO cells. In...
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Article
Isolation and primary culture of adult pig hepatocytes
Hepatocytes were directly isolated from pig livers obtained from the abattoir and cultivated on a type I collagen-coated polystyrene surface. Digestion of the partial liver lobe resulted in an average yield of...
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Chapter
Morphology and Function of Rat Hepatocytes Cultured on Expanded Polytetrafluoroethylene Surface
for the development of a bioreactor using hepatocytes, particularly for a hybrid-type artificial liver, we study culture substrates in this paper with particular attention to a porous material, expanded polyte...
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Article
A method for transforming human liver epithelial cells by transfection using a plasmid containing SV40 early region gene
A method is described for establishing continuous cell lines of liver epithelial cells by transformation of cultured hepatocytes isolated by collagenase/dispase of adult human liver tissue. Between 2 to 5×105 hep...
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Article
Alpha-fetoprotein-producing capacity, chromosomal and morphological properties in human hepatoma cells treated with various chemotherapeutic agents
Alpha-fetoprotein (AFP)-producing capacity and some other properties of human hepatoma cell lines treated with chemotherapeutic agents, such as mitomycin C, Adriamycin, cisplatinum, and 5-fluorouracil were inv...
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Article
Effects of chemotherapeutic agents on alpha-fetoprotein secretion and growth of human hepatoma cell lines in vitro
The effects of various chemotherapeutic agents on alpha-fetoprotein (AFP) secretion and growth of human hepatocellular carcinoma and hepatoblastoma cell lines were investigated in vitro. It was found that ther...
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Article
Establishment and characterization of a cell line from a human cholangiocellular carcinoma
A cell line, HuH-28, was established in vitro from a patient with cholangiocellular carcinoma (CCC). This cell line has grown slowly, revealing a doubling time of approximately 80 h, and the serial passages we...
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Article
Isolation of clones from T24 cells and test of their sensitivity to adriamycin
Two single cell clones, CL3 and CL7, were developed from T24 cells. CL3 had abundant cytoplasmic granules, while CL7 did not have as much. CL3 and CL7 exhibited a modal number of chromosomes at 69 and 74, resp...
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Article
The effect of conditioned medium on fetal human liver cells in primary culture
A medium conditioned by rat embryo cultures (RCM) promoted the adhesion of liver cells from human fetuses to plastic dish. Colonies were also formed in primary cultures of the same cells in the presence of RCM...
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Chapter
Asbestos-Associated Chromosomal Changes in Human Mesothelial Cells
Epidemiological studies have established that exposure to asbestos fibers is the primary cause of mesothelioma in the industrialized world (Kannerstein and Churg 1980; Craighead and Mossman 1982). Because the ...
