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Article
The 4D Nucleome: Genome Compartmentalization in an Evolutionary Context
4D nucleome research aims to understand the impact of nuclear organization in space and time on nuclear functions, such as gene expression patterns, chromatin replication, and the maintenance of genome integri...
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Article
Stressechokardiographie und ihre zentrale Rolle in der kardiologischen Diagnostik
Die Stressechokardiographie (SE) stellt eine zentrale kardiologische diagnostische Methode dar. Die SE ist nicht nur bei der Diagnostik und Führung der Patienten mit koronarer Herzkrankheit (KHK) von immenser ...
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Article
Chromosome shattering: a mitotic catastrophe due to chromosome condensation failure
Chromosome shattering has been described as a special form of mitotic catastrophe, which occurs in cells with unrepaired DNA damage. The shattered chromosome phenotype was detected after application of a metha...
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Article
3D-Fluoreszenz-in-situ-Hybridisierung und Zellkernarchitektur
Fluoreszenz-in-situ-Hybridisierung an dreidimensional konservierten Zellkernen (3D-FISH) ist eine effiziente Methode für Untersuchungen zur 3D-Anordnung von Chromatin im Zellkern. Die Zellkernarchitektur stell...
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Article
Open AccessSpatial quantitative analysis of fluorescently labeled nuclear structures: Problems, methods, pitfalls
The vast majority of microscopic data in biology of the cell nucleus is currently collected using fluorescence microscopy, and most of these data are subsequently subjected to quantitative analysis. The analys...
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Article
Microirradiation of cells with energetic heavy ions
The ion microprobe SNAKE at the Munich 14 MV tandem accelerator achieves beam focussing by a superconducting quadrupole doublet and can make use of a broad range of ions and ion energies, from 20 MeV protons t...
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Article
Chromosome territories, nuclear architecture and gene regulation in mammalian cells
The expression of genes is regulated at many levels. Perhaps the area in which least is known is how nuclear organization influences gene expression. Studies of higher-order chromatin arrangements and their dy...
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Article
Structure and dynamics of human interphase chromosome territories in vivo
A new approach is presented which allows the in vivo visualization of individual chromosome territories in the nuclei of living human cells. The fluorescent thymidine analog Cy3-AP3-dUTP was microinjected int...
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Article
The origin of human chromosome 2 analyzed by comparative chromosome map** with a DNA microlibrary
Fluorescencein situ hybridization (FISH) of microlibraries established from distinct chromosome subregions can test the evolutionary conservation of chromosome bands as well as chromosomal rearrangements that occ...
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Chapter
Detection of Genetic Imbalances in Tumor Genomes by Fluorescence in situ Hybridization with Tumor Genomic DNA and Subregional DNA Probes
During the last decade strong evidence has been accumulated for a causal relationship between specific genetic alterations and the abnormal differentiation state and growth behaviour of malignant cells. Such a...
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Article
Evidence for a nuclear compartment of transcription and splicing located at chromosome domain boundaries
The nuclear topography of splicing snRNPs, mRNA transcripts and chromosome domains in various mammalian cell types are described. The visualization of splicing snRNPs, defined by the Sm antigen, and coiled bod...
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Article
Homologies in human and Macasa fuscata chromosomes revealed by in situ suppression hybridization with human chromosome specific DNA libraries
We established chromosomal homologies between all chromosomes of the human karyotype and that of an old world monkey (Macaca fuscata) by chromosomal in situ suppression (CISS) hybridization with human chromosome ...
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Article
Comparative chromosome band map** in primates byin situ suppression hybridization of band specific DNA microlibraries
A DNA-library established from microdissected bands 8q23 to 8q24.1 of normal human chromosomes 8 (Lüdecke et al., 1989) was used as a probe for chromosomal in situ suppression (CISS-) hybridization to metaphas...
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Article
Painting of human chromosomes with probes generated from hybrid cell lines by PCR with Alu and L1 primers
Specific amplification of human sequences of up to several kb length has recently been accomplished in man-hamster and man-mouse somatic hybrid cell DNA by IRS-PCR (interspersed repetitive sequence — polymeras...
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Article
Direct carrier detection by in situ suppression hybridization with cosmid clones of the Duchenne/Becker muscular dystrophy locus
A basic problem in genetic counseling of families with Duchenne/Becker muscular dystrophy (DMD/BMD) concerns the carrier status of female relatives of an affected male. In about 60% of these patients, deletion...
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Article
Chromosomal in situ suppression hybridization of human gonosomes and autosomes and its use in clinical cytogenetics
DNA libraries from sorted human gonosomes were used selectively to stain the X and Y chromosomes in normal and aberrant cultured human cells by chromosomal in situ suppression (CISS-) hybridization. The entire...
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Article
Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries
A method of in situ hybridization for visualizing individual human chromosomes from pter to qter, both in metaphase spreads and interphase nuclei, is reported. DNA inserts from a single chromosomal library are...
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Article
Detection of chromosome aberrations in metaphase and interphase tumor cells by in situ hybridization using chromosome-specific library probes
Chromosome aberrations in two glioma cell lines were analyzed using biotinylated DNA library probes that specifically decorate chromosomes 1, 4, 7, 18 and 22 from pter to qter. Numerical changes, deletions and...
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Article
Detection of chromosome aberrations in the human interphase nucleus by visualization of specific target DNAs with radioactive and non-radioactive in situ hybridization techniques: diagnosis of trisomy 18 with probe L1.84
The localization of chromosome 18 in human interphase nuclei is demonstrated by use of radioactive and nonradioactive in situ hybridization techniques with a DNA clone designated L1.84. This clone represents a...
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Article
Specific staining of human chromosomes in Chinese hamster x man hybrid cell lines demonstrates interphase chromosome territories
In spite of Carl Rabl's (1885) and Theodor Boveri's (1909) early hypothesis that chromosomes occupy discrete territories or domains within the interphase nucleus, evidence in favor pf this hypothesis has been ...
