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Article
Open AccessDiffusion-driven fed-batch fermentation in perforated ring flasks
Simultaneous membrane-based feeding and monitoring of the oxygen transfer rate shall be introduced to the newly established perforated ring flask, which consists of a cylindrical glass flask with an additional...
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Article
Open AccessValidation of computational fluid dynamics of shake flask experiments at moderate viscosity by liquid distributions and volumetric power inputs
Computational fluid dynamics (CFD) has recently become a pivotal tool in the design and scale-up of bioprocesses. While CFD has been extensively utilized for stirred tank reactors (STRs), there exists a relati...
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Article
Open AccessCross-section perimeter is a suitable parameter to describe the effects of different baffle geometries in shaken microtiter plates
Biotechnological screening processes are performed since more than 8 decades in small scale shaken bioreactors like shake flasks or microtiter plates. One of the major issues of such reactors is the sufficient...
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Article
Open AccessDevelopment of a modified Respiration Activity Monitoring System for accurate and highly resolved measurement of respiration activity in shake flask fermentations
The Respiration Activity Monitoring System (RAMOS) is an established device to measure on-line the oxygen transfer rate (OTR), thereby, yielding relevant information about metabolic activities of microorganism...
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Article
Open AccessPotential errors in conventional DOT measurement techniques in shake flasks and verification using a rotating flexitube optical sensor
Dissolved oxygen tension (DOT) is an important parameter for evaluating a bioprocess. Conventional means to measure DOT in shake flasks using fixed Clark-type electrodes immersed in the bulk liquid are problem...
