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Article
Open AccessUSP28 promotes tumorigenesis and cisplatin resistance by deubiquitinating MAST1 protein in cancer cells
Cisplatin is a chemotherapy drug that causes a plethora of DNA lesions and inhibits DNA transcription and replication, resulting in the induction of apoptosis in cancer cells. However, over time, patients deve...
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Article
Deubiquitinase USP19 enhances phenylalanine hydroxylase protein stability and its enzymatic activity
Phenylalanine hydroxylase (PAH) is the key enzyme in phenylalanine metabolism, deficiency of which is associated with the most common metabolic phenotype of phenylketonuria (PKU) and hyperphenylalaninemia (HPA...
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Article
USP19 Negatively Regulates p53 and Promotes Cervical Cancer Progression
p53 is a tumor suppressor gene activated in response to cellular stressors that inhibits cell cycle progression and induces pro-apoptotic signaling. The protein level of p53 is well balanced by the action of s...
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Article
Open AccessCRISPR/Cas9-based genome-wide screening of the deubiquitinase subfamily identifies USP3 as a protein stabilizer of REST blocking neuronal differentiation and promotes neuroblastoma tumorigenesis
The repressor element-1 silencing transcription factor (REST), a master transcriptional repressor, is essential for maintenance, self-renewal, and differentiation in neuroblastoma. An elevated expression of RE...
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Article
Open AccessHuman pluripotent stem cell-derived macrophages and macrophage-derived exosomes: therapeutic potential in pulmonary fibrosis
Pulmonary fibrosis (PF) is a fatal chronic disease characterized by accumulation of extracellular matrix and thickening of the alveolar wall, ultimately leading to respiratory failure. PF is thought to be init...
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Article
Open AccessGenome-wide screening for deubiquitinase subfamily identifies ubiquitin-specific protease 49 as a novel regulator of odontogenesis
Proteins expressed by the paired box gene 9 (PAX9) and Msh Homeobox 1 (MSX1) are intimately involved in tooth development (odontogenesis). The regulation of PAX9 and MSX1 protein turnover by deubiquitinating e...
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Article
Open AccessDeubiquitinase USP19 extends the residual enzymatic activity of phenylalanine hydroxylase variants
Phenylalanine hydroxylase (PAH) is a key enzyme in mammals that maintains the phenylalanine (Phe) concentration at an appropriate physiological level. Some genetic mutations in the PAH gene lead to destabiliza...
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Article
Genome-scale screening of deubiquitinase subfamily identifies USP3 as a stabilizer of Cdc25A regulating cell cycle in cancer
Conventional screening methods for deubiquitinating enzymes (DUBs) have important limitations. A loss-of-function study based on the knockout of DUB genes in mammalian cells can provide an excellent model for ...
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Article
The role of deubiquitinating enzymes in cancer drug resistance
Drug resistance is a well-known phenomenon leading to a reduction in the effectiveness of pharmaceutical treatments. Resistance to chemotherapeutic agents can involve various intrinsic cellular processes inclu...
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Article
Efficient genome editing by FACS enrichment of paired D10A Cas9 nickases coupled with fluorescent proteins
Targeted genome editing by clustered regularly interspaced short palindromic repeats (CRISPR-Cas9) raised concerns over off-target effects. The use of double-nicking strategy using paired Cas9 nickase has been...
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Article
Genome Editing in Stem Cells for Disease Therapeutics
Programmable nucleases including zinc finger nucleases, transcription activator-like effector nucleases, and clustered regularly interspaced short palindrome repeats (CRISPR)/CRISPR-associated protein have tre...
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Article
Genome editing: a robust technology for human stem cells
Human pluripotent stem cells comprise induced pluripotent and embryonic stem cells, which have tremendous potential for biological and therapeutic applications. The development of efficient technologies for th...
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Protocol
Cell-Penetrating Peptide-Mediated Delivery of Cas9 Protein and Guide RNA for Genome Editing
The clustered, regularly interspaced, short palindromic repeat (CRISPR)-associated (Cas) system represents an efficient tool for genome editing. It consists of two components: the Cas9 protein and a guide RNA....
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Article
Open AccessDissecting microRNA-mediated regulation of stemness, reprogramming, and pluripotency
Increasing evidence indicates that microRNAs (miRNAs), endogenous short non-coding RNAs 19–24 nucleotides in length, play key regulatory roles in various biological events at the post-transcriptional level. Em...
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Article
The role of deubiquitinating enzymes in spermatogenesis
Spermatogenesis is a complex process through which spermatogonial stem cells undergo mitosis, meiosis, and cell differentiation to generate mature spermatozoa. During this process, male germ cells experience s...
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Article
Recent developments and clinical studies utilizing engineered zinc finger nuclease technology
Efficient methods for creating targeted genetic modifications have long been sought for the investigation of gene function and the development of therapeutic modalities for various diseases, including genetic ...
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Article
An electrochemical, in vitro bioactivity, and quantum chemical approach to nanostructured copolymer coatings for orthopedic applications
Conducting polymers represent a promising platform toward coating materials for implant technologies in recent years. In this investigation, copolymers based on pyrrole (Py) and 3,4-ethylenedioxythiophene (EDO...
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Article
Surrogate reporter-based enrichment of cells containing RNA-guided Cas9 nuclease-induced mutations
RNA-guided endonucleases (RGENs), which are based on the clustered, regularly interspaced, short palindromic repeat (CRISPR)-CRISPR-associated (Cas) system, have recently emerged as a simple and efficient tool...
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Article
The role of deubiquitinating enzymes in apoptosis
It has become apparent that ubiquitination plays a critical role in cell survival and cell death. In addition, deubiquitinating enzymes (DUBs) have been determined to be highly important regulators of these pr...
