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Article
Open AccessBiohydrogen Production from Food Waste Using Glucose-Adapted Hyperthermophilic Archaeon
Glucose is one of the most important carbon and energy source for heterotrophic growth in all living organisms. However, glucose has been reported as a poor substrate to support the growth of hyperthermophilic...
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Article
Comparison of Intestine Microbiota Between Wild and Farmed Korean Rockfish, Sebastes schlegelii
The Korean rockfish, Sebastes schlegeli, is most commonly farmed in sea cages along the coast of Korea; however, detailed information on intestinal microbiota regarding this fish is not readily available. In this...
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Article
Long-term Operation of Continuous Culture of the Hyperthermophilic archaeon, Thermococcus onnurineus for Carbon Monoxide-dependent Hydrogen Production
In this study, we performed a kinetic analysis of CO-dependent H2 production by metabolically engineered Thermococcus onnurineus NA1, MC01 in terms of the cell activity as well as mass transfer rate. We conducted...
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Article
Thermococcus indicus sp. nov., a Fe(III)-reducing hyperthermophilic archaeon isolated from the Onnuri Vent Field of the Central Indian Ocean ridge
A strictly anaerobic, dissimilatory Fe(III)-reducing hyperthermophilic archaeon, designated as strain IOH1T, was isolated from a new deep-sea hydrothermal vent (Onnuri Vent Field) area in the Central Indian Ocean...
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Article
Paradesulfovibrio onnuriensis gen. nov., sp. nov., a chemolithoautotrophic sulfate-reducing bacterium isolated from the Onnuri vent field of the Indian Ocean and reclassification of Desulfovibrio senegalensis as Paradesulfovibrio senegalensis comb. nov.
An anaerobic, rod-shaped, mesophilic, chemolithoautotrophic, sulfate-reducing bacterial strain IOR2T was isolated from a newly found deep-sea hydrothermal vent (OVF, Onnuri Vent Field) area in the central Indian ...
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Article
MEST induces Twist-1-mediated EMT through STAT3 activation in breast cancers
The loss of imprinting of MEST has been linked to certain types of cancer by promoter switching. However, MEST-mediated regulation of tumorigenicity and metastasis are yet to be understood. Herein, we reported...
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Article
Characterization of the copper-sensing transcriptional regulator CopR from the hyperthermophilic archeaon Thermococcus onnurineus NA1
A putative copper ion-sensing transcriptional regulator CopR (TON_0836) from Thermococcus onnurineus NA1 was characterized. The CopR protein consists of a winged helix-turn-helix DNA-binding domain in the amino-t...
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Article
Formulation of a Low-cost Medium for Improved Cost-effectiveness of Hydrogen Production by Thermococcus onnurineus NA1
Hydrogen has been receiving considerable attention as a clean energy carrier. Thermococcus onnurineus NA1, a hyperthermophilic archaeon, is capable of producing hydrogen using carbon monoxide (CO) in a growth-ass...
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Article
Isolation, cultivation, and genome analysis of proteorhodopsin-containing SAR116-clade strain Candidatus Puniceispirillum marinum IMCC1322
Strain IMCC1322 was isolated from a surface water sample from the East Sea of Korea. Based on 16S rRNA analysis, IMCC1322 was found to belong to the OCS28 sub-clade of SAR116. The cells appeared as short vibri...
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Article
A rapid and sensitive enzymatic assay for 2,3-butanediol
In this study, we developed a rapid and sensitive enzymatic assay for 2,3-butanediol (2,3-BDO) detection. The concentration of 2,3-BDO was determined by measuring the reduction of NADP+ using Clostridium ljungdah...
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Article
A simple biosynthetic pathway for 2,3-butanediol production in Thermococcus onnurineus NA1
The biosynthetic pathway of 2,3-butanediol (2,3-BDO) production from pyruvate under anaerobic conditions includes three enzymes: acetolactate synthase (ALS), acetolactate decarboxylase (ALDC), and acetoin redu...
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Article
Open AccessBiohydrogen production of obligate anaerobic archaeon Thermococcus onnurineus NA1 under oxic conditions via overexpression of frhAGB-encoding hydrogenase genes
The production of biohydrogen (H2) as a promising future fuel in anaerobic hyperthermophiles has attracted great attention because H2 formation is more thermodynamically feasible at elevated temperatures and fewe...
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Article
Oxygen-mediated growth enhancement of an obligate anaerobic archaeon Thermococcus onnurineus NA1
Thermococcus onnurineus NA1, an obligate anaerobic hyperthermophilic archaeon, showed variable oxygen (O2) sensitivity depending on the types of substrate employed as an energy source. Unexpectedly, the culture w...
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Article
Open AccessAdaptive evolution of a hyperthermophilic archaeon pinpoints a formate transporter as a critical factor for the growth enhancement on formate
Previously, we reported that the hyperthermophilic archaeon Thermococcus onnurineus NA1 could grow on formate and produce H2. Formate conversion to hydrogen was mediated by a formate-hydrogen lyase complex and wa...
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Article
Transcriptomic profiling and its implications for the H2 production of a non-methanogen deficient in the frhAGB-encoding hydrogenase
The F420-reducing hydrogenase of methanogens functions in methanogenesis by providing reduced coenzyme F420 (F420H2) as an electron donor. In non-methanogens, however, their physiological function has not been id...
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Article
Redox regulation of SurR by protein disulfide oxidoreductase in Thermococcus onnurineus NA1
Protein disulfide oxidoreductases are redox enzymes that catalyze thiol–disulfide exchange reactions. These enzymes include thioredoxins, glutaredoxins, protein disulfide isomerases, disulfide bond formation A...
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Article
Open AccessGenome-wide primary transcriptome analysis of H2-producing archaeon Thermococcus onnurineus NA1
In spite of their pivotal roles in transcriptional and post-transcriptional processes, the regulatory elements of archaeal genomes are not yet fully understood. Here, we determine the primary transcriptome of ...
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Article
Metagenomic analysis reveals the contribution of anaerobic methanotroph-1b in the oxidation of methane at the Ulleung Basin, East Sea of Korea
We have previously identified a sulfate methane transition zone (SMTZ) within the methane hydrate-bearing sediment in the Ulleung Basin, East Sea of Korea, and the presence of ANME-1b group in the sediment has...
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Article
Phylogenetic relationship between symbionts of tubeworm Lamellibrachia satsuma and the sediment microbial community in Kagoshima Bay
Vestimentiferan tubeworms acquire their symbionts through horizontal transmission from the surrounding environment. In the present study, we constructed a 16S rRNA gene clone library to investigate the phyloge...
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Article
Open AccessA novel family VIII carboxylesterase hydrolysing third- and fourth-generation cephalosporins
A metagenomic library was constructed from a soil sample of spindle tree-rhizosphere. From this library, one clone with esterase activity was selected. The sequence analysis revealed an open reading frame (Est...
