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Article
Open AccessHub stability in the calcium calmodulin-dependent protein kinase II
The calcium calmodulin protein kinase II (CaMKII) is a multi-subunit ring assembly with a central hub formed by the association domains. There is evidence for hub polymorphism between and within CaMKII isoform...
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Article
Open AccessBinary-FRET reveals transient excited-state structure associated with activity-dependent CaMKII - NR2B binding and adaptation
Synaptic functions are mediated and modulated by a coordinated choreography of protein conformational changes and interactions in response to intracellular calcium dynamics. Time-lapse Förster resonance energy...
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FRET as a biomolecular research tool — understanding its potential while avoiding pitfalls
The applications of Förster resonance energy transfer (FRET) grow with each year. However, different FRET techniques are not applied consistently, nor are results uniformly presented, which makes implementing ...
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Article
Open AccessAuto-FPFA: An Automated Microscope for Characterizing Genetically Encoded Biosensors
Genetically encoded biosensors function by linking structural change in a protein construct, typically tagged with one or more fluorescent proteins, to changes in a biological parameter of interest (such as ca...
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Chapter
An Introduction to Interpreting Time Resolved Fluorescence Anisotropy Curves
The decay in fluorescence anisotropy following an excitation pulse can be monitored using time-correlated single photon counting, and used to measure molecular rotation and homo-FRET in biomedical research. In...
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Deep brain optical measurements of cell type–specific neural activity in behaving mice
A description of how to use a time-correlated single-photon counting–based fiber optics system to measure the intensity, emission spectra and lifetime of fluorescent biosensors expressed in deep brain structur...
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Concurrent activation of striatal direct and indirect pathways during action initiation
In mice performing an operant task, increases in neural activity in direct- and indirect-pathway spiny projection neurons (SPNs) are associated with action initiation but not with inactivity, and concurrent ac...
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Photobleaching of YFP does not produce a CFP-like species that affects FRET measurements
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Defective membrane repair in dysferlin-deficient muscular dystrophy
Muscular dystrophy includes a diverse group of inherited muscle diseases characterized by wasting and weakness of skeletal muscle1. Mutations in dysferlin are linked to two clinically distinct muscle diseases, li...
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Article
Application of a Membrane Fusion Assay for Rapid Drug Screening
Purpose. The purpose of this study is to develop an in vitro assay for screening drug and their effects on membrane fusion and lysis of intracellular organelles.
