13 Result(s)
within
Stephen M. Beverley
Life Sciences
-
Article
The Evolutionary Loss of RNAi Key Determinants in Kinetoplastids as a Multiple Sporadic Phenomenon
We screened the genomes of a broad panel of kinetoplastid protists for genes encoding proteins associated with the RNA interference (RNAi) system using probes from the Argonaute (AGO1), Dicer1 (DCL1), and Dice...
-
Article
Open AccessComparative genomic analysis of Leishmania (Viannia) peruviana and Leishmania (Viannia) braziliensis
The Leishmania (Viannia) braziliensis complex is responsible for most cases of New World tegumentary leishmaniasis. This complex includes two closely related species but with different geographic distribution and...
-
Article
Open AccessREDHORSE-REcombination and Double crossover detection in Haploid Organisms using next-geneRation SEquencing data
Next-generation sequencing technology provides a means to study genetic exchange at a higher resolution than was possible using earlier technologies. However, this improvement presents challenges as the alignm...
-
Protocol
A Transposon-Based Tool for Transformation and Mutagenesis in Trypanosomatid Protozoa
The ability of transposable elements to mobilize across genomes and affect the expression of genes makes them exceptional tools for genetic manipulation methodologies. Several transposon-based systems have bee...
-
Article
A transposon toolkit for gene transfer and mutagenesis in protozoan parasites
Protozoan parasites affect millions of people around the world. Treatment and control of these diseases are complicated partly due to the intricate biology of these organisms. The interactions of species of Plasm...
-
Chapter
Sphingolipids in Parasitic Protozoa
The surface of most protozoan parasites relies heavily upon lipid-anchored molecules, to form protective barriers and play critical functions required for infectivity. Sphingolipids (SLs) play impo...
-
Article
PTR1-dependent synthesis of tetrahydrobiopterin contributes to oxidant susceptibility in the trypanosomatid protozoan parasite Leishmania major
Leishmania must survive oxidative stress, but lack many classical antioxidant enzymes and rely heavily on trypanothione-dependent pathways. We used forward genetic screens to recover loci...
-
Protocol
In Vitro Shuttle Mutagenesis Using Engineered Mariner Transposons
Advances in our understanding of the protozoan parasite Leishmania have been facilitated by the development of molecular and genetic tools. One powerful approach for gene identification and analysis is transposon...
-
Article
Dual role of the fringe connection gene in both heparan sulphate and fringe-dependent signalling events
The precise regulation of growth factor signalling is crucial to the molecular control of development in Drosophila. Post-translational modification of signalling molecules is one of the mechanisms that modulate ...
-
Article
Pteridine reductase mechanism correlates pterin metabolism with drug resistance in trypanosomatid parasites
Pteridine reductase (PTR1) is a short-chain reductase (SDR) responsible for the salvage of pterins in parasitic trypanosomatids. PTR1 catalyzes the NADPH-dependent two-step reduction of oxidized pterins to the...
-
Protocol
Transfection ofLeishmania and Trypanosoma brucei by Electroporation
DNA transfection is the introduction of molecular constructs into parasites in a manner permitting the expression of encoded genes. Transfection is an exceedingly powerful technique for analyzing gen etic regu...
-
Article
Molecular evolution inDrosophila and the higher diptera
In this paper, we examine first the steadiness of the rate of evolutionary change in a larval hemolymph protein, LHP, in numerousDrosophila species. We estimated amino acid sequence divergence from immunological ...
-
Article
Molecular evolution inDrosophila and higher diptera
LHP is a suitable protein for studying evolution in flies (Diptera). This blood protein, which occurs at high concentration late in larval development, was purified to homogeneity from 5 species of Drosophilid...
