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Article
Evaluation of circulating leukocyte transcriptome and its relationship with immune function and blood markers in dairy cows during the transition period
Dairy cows during the transition period are faced with important physiological changes which include a dysfunctional immune system and an increased inflammatory state. New data are necessary to understand the ...
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Article
Assessment of simple marker-free genetic transformation techniques in alfalfa
Methods to avoid the presence of selectable marker genes (SMG) in transgenic plants are available but not implemented in many crop species. We assessed the efficiency of simple marker-free Agrobacterium-mediated ...
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Article
Isolation of genes from female sterile flowers in Medicago sativa
A better knowledge of female sporogenesis and gametogenesis could have several practical applications, from commercial hybrid seed production to gene containment in GM crops. With the purpose of isolating gene...
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Article
Open AccessExercise induced stress in horses: Selection of the most stable reference genes for quantitative RT-PCR normalization
Adequate stress response is a critical factor during athlete horses' training and is central to our capacity to obtain better performances while safeguarding animal welfare.
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Article
Non-antibiotic, efficient selection for alfalfa genetic engineering
A selectable marker gene (SMG), usually conferring resistance to an antibiotic or herbicide, is generally introduced into the plant cells with the gene(s) for the trait of interest to allow only the cells that...