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Article
Open AccessGenome-wide epigenetic modifications in sports horses during training as an adaptation phenomenon
With his bicentennial breeding history based on athletic performance, the Thoroughbred horse can be considered the equine sport breed. Although genomic and transcriptomic tools and knowledge are at the state o...
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Article
Open AccessShedding light on cashmere goat hair follicle biology: from morphology analyses to transcriptomic landascape
Cashmere goat is known for its precious undercoat. Being photoperiod-dictated, cashmere growth has been studied focusing mainly on hair follicle cycle phases (anagen, catagen and telogen). An accurate molecula...
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Article
Evaluation of circulating leukocyte transcriptome and its relationship with immune function and blood markers in dairy cows during the transition period
Dairy cows during the transition period are faced with important physiological changes which include a dysfunctional immune system and an increased inflammatory state. New data are necessary to understand the ...
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Article
Open AccessPANEV: an R package for a pathway-based network visualization
During the last decade, with the aim to solve the challenge of post-genomic and transcriptomic data mining, a plethora of tools have been developed to create, edit and analyze metabolic pathways. In particular...
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Protocol
MCSeEd (Methylation Context Sensitive Enzyme ddRAD): A New Method to Analyze DNA Methylation
Methylation context sensitive enzyme ddRAD (MCSeEd) is a NGS-based method for genome-wide investigations of DNA methylation at different contexts requiring only low to moderate sequencing depth. It is particul...
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Article
Open AccessMethylation content sensitive enzyme ddRAD (MCSeEd): a reference-free, whole genome profiling system to address cytosine/adenine methylation changes
Methods for investigating DNA methylation nowadays either require a reference genome and high coverage, or investigate only CG methylation. Moreover, no large-scale analysis can be performed for N6-methyladenosin...
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Article
Open AccessDietary supplementation with olive mill wastewaters induces modifications on chicken jejunum epithelial cell transcriptome and modulates jejunum morphology
The Mediterranean diet is considered one of the healthier food habits and olive oil is one of its key components. Olive oil polyphenols are known to induce beneficial effects in several pathological conditions...
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Article
Open AccessGene co-expression networks in liver and muscle transcriptome reveal sex-specific gene expression in lambs fed with a mix of essential oils
Essential oil (EO) dietary supplementation is a new strategy to improve animal health. EO compounds have antiparasitic, antimicrobial, antiviral, antimycotic, antioxidant and anti-inflammatory proprieties. Nut...
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Article
Open AccessTranscriptome Analysis of Canine Cutaneous Melanoma and Melanocytoma Reveals a Modulation of Genes Regulating Extracellular Matrix Metabolism and Cell Cycle
Interactions between tumor cells and tumor microenvironment are considered critical in carcinogenesis, tumor invasion and metastasis. To examine transcriptome changes and to explore the relationship with tumor...
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Article
Genetic and phenotypic characterization of the Maremmano horse stud in Castelporziano
The Maremmano is an indigenous Italian horse probably descending from the native breed of the Etruscans (800 BC) bred in Maremma areal that spans from Southern Tuscany to Northern Latium. The Maremmano breed r...
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Article
Open AccessFirst report of junctional epidermolysis bullosa (JEB) in the Italian draft horse
Epitheliogenesis imperfecta in horses was first recognized at the beginning of the 20th century when it was proposed that the disease could have a genetic cause and an autosomal recessive inheritance pattern. ...
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Article
Variation of DNA methylation and phenotypic traits following unilateral sexual polyploidization in Medicago
Sexual hybridization is an important generator of biodiversity and a powerful breeding tool. Hybridization can also overcome ploidy barriers when it involves 2n gametes, as in the case of unilateral sexual polypl...
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Article
Assessment of simple marker-free genetic transformation techniques in alfalfa
Methods to avoid the presence of selectable marker genes (SMG) in transgenic plants are available but not implemented in many crop species. We assessed the efficiency of simple marker-free Agrobacterium-mediated ...
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Article
Open AccessAthletic humans and horses: Comparative analysis of interleukin-6 (IL-6) and IL-6 receptor (IL-6R) expression in peripheral blood mononuclear cells in trained and untrained subjects at rest
Horses and humans share a natural proclivity for athletic performance. In this respect, horses can be considered a reference species in studies designed to optimize physical training and disease prevention. In...
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Article
Age-dependent prevalence of equid herpesvirus 5 infection
Equid herpesvirus 5 (EHV-5) infection was detected in a farm in Italy by the use of a semi-nested polymerase chain reaction (PCR) targeting glycoprotein B of EHV-5 on nasal swabs and blood samples of clinicall...
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Chapter and Conference Paper
Polyploidization and Gene Expression in Medicago sativa
Polyploidization is a common event in plant evolution and can influence economically important traits. Modifications of gene expression and/or DNA sequence are known to occur as a consequence of polyploidizati...
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Article
Open AccessExercise-induced up-regulation of MMP-1 and IL-8 genes in endurance horses
The stress response is a critical factor in the training of equine athletes; it is important for performance and for protection of the animal against physio-pathological disorders.
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Article
Isolation of genes from female sterile flowers in Medicago sativa
A better knowledge of female sporogenesis and gametogenesis could have several practical applications, from commercial hybrid seed production to gene containment in GM crops. With the purpose of isolating gene...
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Article
Open AccessExercise induced stress in horses: Selection of the most stable reference genes for quantitative RT-PCR normalization
Adequate stress response is a critical factor during athlete horses' training and is central to our capacity to obtain better performances while safeguarding animal welfare.
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Article
Non-antibiotic, efficient selection for alfalfa genetic engineering
A selectable marker gene (SMG), usually conferring resistance to an antibiotic or herbicide, is generally introduced into the plant cells with the gene(s) for the trait of interest to allow only the cells that...
