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Article
Differential quantitative analysis of MHC ligands by mass spectrometry using stable isotope labeling
Currently, no method allows direct and quantitative comparison of MHC-presented peptides in pairs of samples, such as transfected and untransfected, tumorous and normal or infected and uninfected tissues or ce...
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Protocol
SYFPEITHI
Reverse immunology has been used for about 12 years in order to identify T-cell epitopes from pathogens or tumor-associated antigens. In this chapter, we discuss the advantages and pitfalls of T-cell epitope p...