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Article
SARS-CoV-2-derived peptides define heterologous and COVID-19-induced T cell recognition
T cell immunity is central for the control of viral infections. To characterize T cell immunity, but also for the development of vaccines, identification of exact viral T cell epitopes is fundamental. Here we ...
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Article
Open AccessIntegrative -omics and HLA-ligandomics analysis to identify novel drug targets for ccRCC immunotherapy
Clear cell renal cell carcinoma (ccRCC) is the dominant subtype of renal cancer. With currently available therapies, cure of advanced and metastatic ccRCC is achieved only in rare cases. Here, we developed a w...
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Article
Open AccessMass spectrometry-based identification of a B-cell maturation antigen-derived T-cell epitope for antigen-specific immunotherapy of multiple myeloma
The B-cell maturation antigen (BCMA) is currently being evaluated as promising tumor-associated surface antigen for T-cell-based immunotherapy approaches, such as CAR T cells and bispecific antibodies, in mult...
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Article
Open AccessA new synthetic toll-like receptor 1/2 ligand is an efficient adjuvant for peptide vaccination in a human volunteer
We previously showed that the bacterial lipopeptide Pam3Cys-Ser-Ser, meanwhile established as a toll-like receptor (TLR) 1/2 ligand, acts as a strong adjuvant for the induction of virus specific CD8+ T cells in m...
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Article
Open AccessMulti-omics discovery of exome-derived neoantigens in hepatocellular carcinoma
Although mutated HLA ligands are considered ideal cancer-specific immunotherapy targets, evidence for their presentation is lacking in hepatocellular carcinomas (HCCs). Employing a unique multi-omics approach ...
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Article
Publisher Correction: Actively personalized vaccination trial for newly diagnosed glioblastoma
The additional author support information was erroneously omitted from the Supplementary Information. This has been corrected online.
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Protocol
Purification and Identification of Naturally Presented MHC Class I and II Ligands
The large-scale and in-depth identification of MHC class I- and II-presented peptides is indispensable for gaining insight into the fundamental rules of immune recognition as well as for develo** innovative ...
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Protocol
Identification of MHC Ligands and Establishing MHC Class I Peptide Motifs
MHC class I peptide motifs are used on a regular basis to identify and predict MHC class I ligands and CD8+ T cell epitopes. This approach is above all an invaluable tool for the identification of disease-associa...
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Article
Actively personalized vaccination trial for newly diagnosed glioblastoma
Patients with glioblastoma currently do not sufficiently benefit from recent breakthroughs in cancer treatment that use checkpoint inhibitors1,2. For treatments using checkpoint inhibitors to be successful, a hig...
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Article
The natural HLA ligandome of glioblastoma stem-like cells: antigen discovery for T cell-based immunotherapy
Glioblastoma is the most frequent malignant primary brain tumor. In a hierarchical tumor model, glioblastoma stem-like cells (GSC) play a major role in tumor initiation and maintenance as well as in therapy re...
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Article
A combined approach of human leukocyte antigen ligandomics and immunogenicity analysis to improve peptide-based cancer immunotherapy
The breakthrough development of immune checkpoint inhibitors as clinically effective novel therapies demonstrates the potential of cancer immunotherapy. The identification of suitable targets for specific immu...
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Article
Data analysis as a source of variability of the HLA-peptide multimer assay: from manual gating to automated recognition of cell clusters
Multiparameter flow cytometry is an indispensable method for assessing antigen-specific T cells in basic research and cancer immunotherapy. Proficiency panels have shown that cell sample processing, test proto...
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Article
A vaccine targeting mutant IDH1 induces antitumour immunity
The mutant IDH1 protein, which is expressed in a large fraction of human gliomas, is shown to be immunogenic; mutant-specific immune responses can be detected in patients with IDH1 mutated gliomas and generate...
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Article
Open AccessTargeted therapy in renal cell carcinoma: moving from molecular agents to specific immunotherapy
Non-specific immunotherapy has been for a long time a standard treatment option for patients with metastatic renal cell carcinoma but was redeemed by specific targeted molecular therapies, namely the VEGF and ...
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Article
Identification of HLA ligands and T-cell epitopes for immunotherapy of lung cancer
Lung cancer is the most common cancer worldwide. Every year, as many people die of lung cancer as of breast, colon and rectum cancers combined. Because most patients are being diagnosed in advanced, not resect...
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Article
Open AccessMouse urinary peptides provide a molecular basis for genotype discrimination by nasal sensory neurons
Selected groups of peptides, including those that are presented by major histocompatibility complex (MHC) proteins, have been proposed to transmit information to the olfactory system of vertebrates via their a...
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Article
Natural HLA class I ligands from glioblastoma: extending the options for immunotherapy
Glioblastoma multiforme is the most frequent and most malignant primary brain tumor with poor prognosis despite surgical removal and radio-chemotherapy. In this setting, immunotherapeutical strategies have gre...
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Protocol
Establishing MHC Class I Peptide Motifs
Major histocompatibility complex (MHC) class I peptide motifs are used on a regular basis to identify and predict MHC class I ligands and CD8+ T-cell epitopes. This approach is above all an invaluable tool for th...
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Protocol
Biochemical Large-Scale Identification of MHC Class I Ligands
The large-scale identification of MHC class I presented peptides is indispensable for gaining insight into the fundamental rules of immune recognition as well as it is an invaluable tool in identifying potenti...
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Article
Insights into MHC class I antigen processing gained from large-scale analysis of class I ligands
Short peptides derived from intracellular proteins and presented on MHC class I molecules on the cell surface serve as a showcase for the immune system to detect pathogenic or malignant alterations inside the ...
