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Article
Open AccessMig1 localization exhibits biphasic behavior which is controlled by both metabolic and regulatory roles of the sugar kinases
Glucose, fructose and mannose are the preferred carbon/energy sources for the yeast Saccharomyces cerevisiae. Absence of preferred energy sources activates glucose derepression, which is regulated by the kinase S...
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Applying Microfluidic Systems to Study Effects of Glucose at Single-Cell Level
Microfluidic systems in combination with microscopy (e.g., fluorescence) can be a powerful tool to study, at single-cell level, the behavior and morphology of biological cells after uptake of glucose. Here, we...
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Article
Open AccessSingle-cell study links metabolism with nutrient signaling and reveals sources of variability
The yeast AMPK/SNF1 pathway is best known for its role in glucose de/repression. When glucose becomes limited, the Snf1 kinase is activated and phosphorylates the transcriptional repressor Mig1, which is then ...
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Article
Editor’s comment on “CRISPR/Cas9-mediated gene editing in human zygotes using Cas9 protein”
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Article
Open AccessThe yeast osmostress response is carbon source dependent
Adaptation to altered osmotic conditions is a fundamental property of living cells and has been studied in detail in the yeast Saccharomyces cerevisiae. Yeast cells accumulate glycerol as compatible solute, contr...
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Moving from Genetics to Systems Biology
Access to competence in mathematical modelling may be a major hindrance for employing a systems biology approach in many research projects. Very commonly, the most successful systems biology studies are based ...
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Article
Open AccessSystems Level Analysis of the Yeast Osmo-Stat
Adaptation is an important property of living organisms enabling them to cope with environmental stress and maintaining homeostasis. Adaptation is mediated by signaling pathways responding to different stimuli...
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Expression of concern: Sequences enhancing cassava mosaic disease symptoms occur in the cassava genome and are associated with South African cassava mosaic virus infection by A. T. Maredza, F. Allie, G. Plata, M. E. C. Rey
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Open AccessStrategies for structuring interdisciplinary education in Systems Biology: an European perspective
Systems Biology is an approach to biology and medicine that has the potential to lead to a better understanding of how biological properties emerge from the interaction of genes, proteins, molecules, cells and...
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Article
Open AccessNetwork reconstruction and validation of the Snf1/AMPK pathway in baker’s yeast based on a comprehensive literature review
The SNF1/AMPK protein kinase has a central role in energy homeostasis in eukaryotic cells. It is activated by energy depletion and stimulates processes leading to the production of ATP while it downregulates A...
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Article
An integrated view on a eukaryotic osmoregulation system
Osmoregulation encompasses active homeostatic processes that ensure proper cell volume, shape and turgor as well as an intercellular milieu optimal for the diverse biochemical processes. Recent studies demonst...
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Bridging the gaps in systems biology
Systems biology aims at creating mathematical models, i.e., computational reconstructions of biological systems and processes that will result in a new level of understanding—the elucidation of the basic and ...
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Article
Open AccessRewiring yeast osmostress signalling through the MAPK network reveals essential and non-essential roles of Hog1 in osmoadaptation
Mitogen-activated protein kinases (MAPKs) have a number of targets which they regulate at transcriptional and post-translational levels to mediate specific responses. The yeast Hog1 MAPK is essential for cell ...
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Article
A novel chloroplast localized Rab GTPase protein CPRabA5e is involved in stress, development, thylakoid biogenesis and vesicle transport in Arabidopsis
A novel Rab GTPase protein in Arabidopsis thaliana, CPRabA5e (CP = chloroplast localized) is located in chloroplasts and has a role in transport. Transient expression of CPRabA5e:EGFP fusion protein in tobacco (N...
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Article
Open AccessTime course gene expression profiling of yeast spore germination reveals a network of transcription factors orchestrating the global response
Spore germination of the yeast Saccharomyces cerevisiae is a multi-step developmental path on which dormant spores re-enter the mitotic cell cycle and resume vegetative growth. Upon addition of a fermentable carb...
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Article
Distributed biological computation with multicellular engineered networks
For the creativity of synthetic biologists to be unleashed, basic circuits must become truly interchangeable — that is, modular and scalable. Two papers in this week's Nature take steps towards that goal — one fr...
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Article
Open AccessBiophysical properties of Saccharomyces cerevisiae and their relationship with HOG pathway activation
Parameterized models of biophysical and mechanical cell properties are important for predictive mathematical modeling of cellular processes. The concepts of turgor, cell wall elasticity, osmotically active vol...
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Article
One hundred years of Molecular Genetics and Genomics: 100 years of extra-nuclear inheritance
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Saccharomyces cerevisiae Spore Germination
Saccharomyces cerevisiae spore germination is the process in which dormant spores resume growth. Upon exposure to glucose and other essential nutrients, the spore gradually loses its spore characte...
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Chapter
Hog1-mediated Metabolic Adjustments Following Hyperosmotic Shock in the Yeast Saccharomyces cerevisiae
Yeast cells exposed to hyperosmotic conditions adjust their metabolism in order to increase the production of the compatible solute glycerol. The MAPK Hog1 seems to affect glycerol production rapidly by st...
