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Article
Open AccessFanzor is a eukaryotic programmable RNA-guided endonuclease
RNA-guided systems, which use complementarity between a guide RNA and target nucleic acid sequences for recognition of genetic elements, have a central role in biological processes in both prokaryotes and euka...
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Article
Open AccessCryo-EM structure of the transposon-associated TnpB enzyme
The class 2 type V CRISPR effector Cas12 is thought to have evolved from the IS200/IS605 superfamily of transposon-associated TnpB proteins1. Recent studies have identified TnpB proteins as miniature RNA-guided D...
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Article
Open AccessStructure of the IscB–ωRNA ribonucleoprotein complex, the likely ancestor of CRISPR-Cas9
Transposon-encoded IscB family proteins are RNA-guided nucleases in the OMEGA (obligate mobile element-guided activity) system, and likely ancestors of the RNA-guided nuclease Cas9 in the type II CRISPR-Cas ad...
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Article
Open AccessStructure of the OMEGA nickase IsrB in complex with ωRNA and target DNA
RNA-guided systems, such as CRISPR–Cas, combine programmable substrate recognition with enzymatic function, a combination that has been used advantageously to develop powerful molecular technologies1,2. Structura...
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Article
Compact RNA editors with small Cas13 proteins
CRISPR–Cas13 systems have been developed for precise RNA editing, and can potentially be used therapeutically when temporary changes are desirable or when DNA editing is challenging. We have identified and cha...