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Article
Open AccessStructure and engineering of Brevibacillus laterosporus Cas9
The RNA-guided DNA endonuclease Cas9 cleaves double-stranded DNA targets complementary to an RNA guide, and is widely used as a powerful genome-editing tool. Here, we report the crystal structure of Brevibacillus...
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Article
Open AccessStructural mechanism of bridge RNA-guided recombination
Insertion sequence (IS) elements are the simplest autonomous transposable elements found in prokaryotic genomes1. We recently discovered that IS110 family elements encode a recombinase and a non-coding bridge RNA...
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Article
Open AccessStructure of the IscB–ωRNA ribonucleoprotein complex, the likely ancestor of CRISPR-Cas9
Transposon-encoded IscB family proteins are RNA-guided nucleases in the OMEGA (obligate mobile element-guided activity) system, and likely ancestors of the RNA-guided nuclease Cas9 in the type II CRISPR-Cas ad...
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Article
Open AccessEngineered Campylobacter jejuni Cas9 variant with enhanced activity and broader targeting range
The RNA-guided DNA endonuclease Cas9 is a versatile genome-editing tool. However, the molecular weight of the commonly used Streptococcus pyogenes Cas9 is relatively large. Consequently, its gene cannot be effici...
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Article
Open AccessAmplification-free RNA detection with CRISPR–Cas13
CRISPR-based nucleic-acid detection is an emerging technology for molecular diagnostics. However, these methods generally require several hours and could cause amplification errors, due to the pre-amplificatio...
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Article
Crystal structure of heliorhodopsin
Heliorhodopsins (HeRs) are a family of rhodopsins that was recently discovered using functional metagenomics1. They are widely present in bacteria, archaea, algae and algal viruses2,3. Although HeRs have seven pr...
