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Article
Robust organ size requires robust timing of initiation orchestrated by focused auxin and cytokinin signalling
Organ size and shape are precisely regulated to ensure proper function. The four sepals in each Arabidopsis thaliana flower must maintain the same size throughout their growth to continuously enclose and protect ...
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Protocol
From Stained Plant Tissues to Quantitative Cell Segmentation Analysis with MorphoGraphX
Development and growth of plant organs is determined by a myriad of molecular processes that occur in each individual cell. As a direct consequence of these processes, cells alter in size and shape. They there...
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Article
Open Access3DCellAtlas Meristem: a tool for the global cellular annotation of shoot apical meristems
Modern imaging approaches enable the acquisition of 3D and 4D datasets capturing plant organ development at cellular resolution. Computational analyses of these data enable the digitization and analysis of ind...
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Protocol
Quantifying Plant Growth and Cell Proliferation with MorphoGraphX
Confocal microscopy is widely used to live-image plant tissue. Cell outlines can be visualized using fluorescent probes that mark the cell wall or plasma membrane, enabling the confocal microscope to be used a...
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Chapter
Modeling Plant Tissue Growth and Cell Division
Morphogenesis is the creation of form, a complex process requiring the integration of genetics, mechanics, and geometry. Patterning processes driven by molecular regulatory and signaling networks interact with...
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Protocol
In Silico Methods for Cell Annotation, Quantification of Gene Expression, and Cell Geometry at Single-Cell Resolution Using 3DCellAtlas
A comprehensive understanding of plant growth and development requires the integration of the spatial and temporal dynamics of gene regulatory networks with changes in cellular geometry during 3D organ growth....
