36 Result(s)
within
Si** He
Chemistry
Biomedicine, general
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Article
MiR-214 promotes the antitumor effect of NK cells in colorectal cancer liver metastasis through USP27X/Bim
Colorectal cancer (CRC) is a common tumor type, and liver metastasis reduces the long-term survival in CRC patients. Natural killer (NK) cells play an important role in anti-tumor immunity. The aim of this stu...
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Targeting RPL23 restores chemosensitivity of cisplatin-resistant ovarian carcinoma by inhibiting EMT
Chemoresistance is the inevitable outcome of chemotherapy for epithelial ovarian carcinoma (EOC), and its mechanism is still not fully understood. This study explored the role of ribosomal protein L23 (RPL23) ...
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Article
Acetyl l-carnitine protects adipose-derived stem cells against serum-starvation: regulation on the network composed of reactive oxygen species, autophagy, apoptosis and senescence
Adipose-derived stem cells (ADSCs) play an important role in cell therapy and regenerative medicine. However, local nutritional deficiency often limits therapeutical effect of the transplanted cells. Acetyl l-car...
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Substrate coated with autologous decellularized extracellular matrix facilitates in vitro spreading of spheroid from adipose-derived stem cells through regulating ERK1/2-MMP2/9 pathway
Adipose-derived stem cells (ADSCs) are easily available and play an important role in regenerative medicine. In recent years, Cell spheroid models have been in the spotlight because of their various advantages...
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Article
Long non-coding RNA ZFAS1 exerts a protective role to alleviate oxygen and glucose deprivation-mediated injury in ischemic stroke cell model through targeting miR-186-5p/MCL1 axis
In recent years, accumulating articles have revealed that long non-coding RNAs (lncRNAs) play crucial roles in ischemic stroke (IS). A previous study found that lncRNA zinc finger antisense 1 (ZFAS1) was down-...
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Article
Indocyanine Green Uptake and Periodic Acid–Schiff Staining Method for Function Detection of Liver Cells are Affected by Different Cell Confluence
Hepatic stem cell transplantation has been demonstrated as an effective alternative therapy for the end-stage liver failure patients. Therefore, the functional detection of hepatic stem cell is essentially req...
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Article
MiR-29b-3p aggravates cardiac hypoxia/reoxygenation injury via targeting PTX3
Our current research aimed to decipher the role and underlying mechanism with regard to miR-29b-3p involving in myocardial ischemia/reperfusion (I/R) injury. In the present study, cardiomyocyte H9c2 cell was u...
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Article
Production of lentiviral vectors in suspension cells using low proportion of supercoiled circular plasmid DNA
The supercoiled circular (SC) topology form of plasmid DNA has been regarded to be advantageous over open circular or linearized analogue in transfection and expression efficiency, and therefore are largely de...
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Article
Transcription factor Isl1 is dispensable for the development of the mouse prosensory region
In order to identify genes involved in the development of inner ear hair cells, we investigated the role of the transcription factor Islet-class LIM-homeodomain (LIM-HD) 1 (Isl1) in the development of the mous...
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Article
Establishing a dual knock-out cell line by lentivirus based combined CRISPR/Cas9 and Loxp/Cre system
The clustered regulatory interspersed short palindromic repeat (CRISPR)/CRISPR-associated protein (Cas) system has been widely used for gene knock-out. Lentiviral vectors have been commonly used as a delivery ...
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The role of Sox9 in maintaining the characteristics and pluripotency of Arbas Cashmere goat hair follicle stem cells
In our previous work, we isolated Arbas Cashmere goat hair follicle stem cells (gHFSCs) and explored the pluripotency. In this study, we investigated the expression and putative role of Sox9 in the gHFSCs. Imm...
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Article
Development and validation of a simple method for the extraction of human skin melanocytes
Primary melanocytes in culture are useful models for studying epidermal pigmentation and efficacy of melanogenic compounds, or develo** advanced therapy medicinal products. Cell extraction is an inevitable a...
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Article
Therapeutic effect of 28-homobrassinolide on leukotriene synthesis in leukemia cells
The present study was aimed to investigate the effect of 28-homobrassinolide on leukotriene synthesis in the 2H3 cells. Cell viability was determined by using sulforhodamine B (SRB) assay. Leukotriene C4 (LTC4) a...
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Article
Transplantation of mesenchymal stem cells modulated Cx43 and Cx45 expression in rats with myocardial infarction
At present, little is known about the influence of mesenchymal stem cell (MSC) transplantation on connexin43 (Cx43) and connexin45 (Cx45) remodeling in the ischemic heart. In this study, we investigated the ef...
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Article
Molecular mechanisms of Bombyx batryticatus ethanol extract inducing gastric cancer SGC-7901 cells apoptosis
Bombyx batryticatus is a traditional Chinese medicine. To understand apoptotic effect of B. batryticatus ethanol extract (BBE), we investigated the role of BBE in inducing apoptosis of hu...
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Article
Lithium chloride promotes neuronal differentiation of rat neural stem cells and enhances neural regeneration in Parkinson’s disease model
Parkinson’s disease (PD) is one of the most common neural degenerative disease, affecting millions of people globally. Great progress has been made in the PD treatment, and one of the most promising one is the...
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Article
Lactobacillus slpA promotes ESC growth through the ERK1/2 pathway
Bacterial surface layers (S-layers) are cell envelope structures ubiquitously found in gram-negative and gram-positive bacteria, including Lactobacillus. S-layers play a role in the determination and maintenance ...
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Article
Isolation and characterization of hair follicle stem cells from Arbas Cashmere goat
In this study, highly purified hair follicle stem cells from Arbas Cashmere goat (gHFSCs) were isolated using enzyme digestion and adhesion to type IV collagen. The biological characteristics of the gHFSCs wer...
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A study to identify and characterize the stem/progenitor cell in rabbit meniscus
The repair of meniscus in the avascular zone remains a great challenge, largely owing to their limited healing capacity. Stem cells based tissue engineering provides a promising treatment option for damaged me...
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Article
Immortalization of pig fibroblast cells by transposon-mediated ectopic expression of porcine telomerase reverse transcriptase
Pigs are the most economically important livestock, but pig cell lines useful for physiological studies and/or vaccine development are limited. Although several pig cell lines have been generated by oncogene t...
