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Article
Open AccessInvestigation of two metabolic engineering approaches for (R,R)-2,3-butanediol production from glycerol in Bacillus subtilis
Flux Balance Analysis (FBA) is a well-known bioinformatics tool for metabolic engineering design. Previously, we have successfully used single-level FBA to design metabolic fluxes in Bacillus subtilis to enhance ...
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Article
Open AccessEndocrine secretory granule production is caused by a lack of REST and intragranular secretory content and accelerated by PROX1
Endocrine secretory granules (ESGs) are morphological characteristics of endocrine/neuroendocrine cells and store peptide hormones/neurotransmitters. ESGs contain prohormones and ESG-related molecules, mainly ...
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Article
Open AccessDirect 1,3-butadiene biosynthesis in Escherichia coli via a tailored ferulic acid decarboxylase mutant
The C4 unsaturated compound 1,3-butadiene is an important monomer in synthetic rubber and engineering plastic production. However, microorganisms cannot directly produce 1,3-butadiene when glucose is used as a...
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Article
Open AccessPlasmacytoid urothelial carcinoma of renal pelvis with positive zinc finger E–box–binding homeobox 1: a case report
The tumor transformation mechanism of a plasmacytoid urothelial carcinoma remains unexplained. We describe the case of a plasmacytoid urothelial carcinoma of the renal pelvis in which the expression of zinc fi...
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Article
Open AccessPrognostic impact of a tumor-infiltrating lymphocyte subtype in triple negative cancer of the breast
Tumor-infiltrating lymphocytes (TILs) have recently been reported as an important factor in the tumor microenvironment and influence the growth and progression of cancer. However, the relationship between immu...
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Article
Open AccessPublisher Correction: Metabolic engineering of Escherichia coli for shikimate pathway derivative production from glucose-xylose co-substrate
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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Article
Open AccessMetabolic engineering of Escherichia coli for shikimate pathway derivative production from glucose–xylose co-substrate
Glucose and xylose are the major components of lignocellulose. Effective utilization of both sugars can improve the efficiency of bioproduction. Here, we report a method termed parallel metabolic pathway engin...
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Article
Open AccessReconstruction of metabolic pathway for isobutanol production in Escherichia coli
The microbial production of useful fuels and chemicals has been widely studied. In several cases, glucose is used as the raw material, and almost all microbes adopt the Embden–Meyerhof (EM) pathway to degrade ...
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Article
Open AccessEngineering a synthetic pathway for maleate in Escherichia coli
Maleate is one of the most important dicarboxylic acids and is used to produce various polymer compounds and pharmaceuticals. Herein, microbial production of maleate is successfully achieved, to our knowledge ...
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Article
4-Vinylphenol production from glucose using recombinant Streptomyces mobaraense expressing a tyrosine ammonia lyase from Rhodobacter sphaeroides
To find a novel host for the production of 4-vinylphenol (4VPh) by screening Streptomyces species.
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Article
Open AccessSecretory production of tetrameric native full-length streptavidin with thermostability using Streptomyces lividans as a host
Streptavidin is a tetrameric protein derived from Streptomyces avidinii, and has tight and specific biotin binding affinity. Applications of the streptavidin-biotin system have been widely studied. Streptavidin i...
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Article
Open AccessHyper secretion of Thermobifida fusca β-glucosidase via a Tat-dependent signal peptide using Streptomyces lividans
Protein production as secretory-form is a powerful tool in industrial enzyme production due to the simple purification procedure. Streptomyces lividans is a versatile host for secretory production of useful prote...
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Article
Creation of endoglucanase-secreting Streptomyces lividans for enzyme production using cellulose as the carbon source
We screened for high-activity endoglucanase (EG) as a first step toward the creation of cellulose-assimilating Streptomyces lividans transformants. EGs derived from Thermobifida fusca YX, Tfu0901, and S. lividans
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Article
Open Accessp-Hydroxycinnamic acid production directly from cellulose using endoglucanase- and tyrosine ammonia lyase-expressing Streptomyces lividans
p-Hydroxycinnamic acid (pHCA) is an aromatic compound that serves as a starting material for the production of many commercially valuable chemicals, such as fragrances and pharmaceuticals, and is also used in t.....
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Article
Open AccessBenzoic acid fermentation from starch and cellulose via a plant-like β-oxidation pathway in Streptomyces maritimus
Benzoic acid is one of the most useful aromatic compounds. Despite its versatility and simple structure, benzoic acid production using microbes has not been reported previously. Streptomyces are aerobic, Gram-pos...
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Article
Cinnamic acid production using Streptomyces lividans expressing phenylalanine ammonia lyase
Cinnamic acid production was demonstrated using Streptomyces as a host. A gene encoding phenylalanine ammonia lyase (PAL) from Streptomyces maritimus was introduced into Streptomyces lividans, and its expression ...
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Article
Analysis of non-genetic risk factors for adverse skin reactions to radiotherapy among 284 Breast Cancer patients
We analyzed non-genetic risk factors for adverse skin reactions to irradiation at 4 collaborating Japanese institutions, to design future investigation into genetic risk factors for adverse skin reactions to i...
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Article
Keap1-null mutation leads to postnatal lethality due to constitutive Nrf2 activation
Transcription factor Nrf2 (encoded by Nfe2l2) regulates a battery of detoxifying and antioxidant genes, and Keap1 represses Nrf2 function. When we ablated Keap1, Keap1-deficient mice died postnatally, probably fr...
