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  1. Article

    Open Access

    Unveiling the underlying molecular mechanisms of high lutein production efficiency in Chlorella sorokiniana FZU60 under a mixotrophy/photoautotrophy two-stage strategy by transcriptomic, physiological, and biochemical analyses

    Chlorella sorokiniana FZU60 is a promising lutein producing microalga. A mixotrophy/photoautotrophy two-stage strategy can achieve high biomass concentration at stage 1 and high lutein content at stage 2, leading...

    Ruijuan Ma, Zhen Zhang, Hong Fang, in Biotechnology for Biofuels and Bioproducts (2023)

  2. Article

    Open Access

    Using a trait-based approach to optimize mixotrophic growth of the red microalga Porphyridium purpureum towards fatty acid production

    Organic carbon sources have been reported to simultaneously increase the growth and lipid accumulation in microalgae. However, there have been no studies of the mixotrophic growth of Porphyridium purpureum in org...

    Kailin Jiao, Wupeng **ao, Yuanchao Xu, in Biotechnology for Biofuels (2018)

  3. Article

    Open Access

    Cell growth and lipid accumulation of a microalgal mutant Scenedesmus sp. Z-4 by combining light/dark cycle with temperature variation

    The light/dark cycle is one of the most important factors affecting the microalgal growth and lipid accumulation. Biomass concentration and lipid productivity could be enhanced by optimization of light/dark cy...

    Chao Ma, Yan-Bo Zhang, Shih-Hsin Ho, De-Feng **ng, Nan-Qi Ren in Biotechnology for Biofuels (2017)

  4. Article

    Open Access

    Feasibility of CO2 mitigation and carbohydrate production by microalga Scenedesmus obliquus CNW-N used for bioethanol fermentation under outdoor conditions: effects of seasonal changes

    Although outdoor cultivation systems have been widely used for mass production of microalgae at a relatively low cost, there are still limited efforts on outdoor cultivation of carbohydrate-rich microalgae tha...

    Shih-Hsin Ho, Yi-Di Chen, Ching-Yu Chang, Yen-Ying Lai in Biotechnology for Biofuels (2017)

  5. Article

    Open Access

    Rapid in vivo lipid/carbohydrate quantification of single microalgal cell by Raman spectral imaging to reveal salinity-induced starch-to-lipid shift

    Lipid/carbohydrate content and ratio are extremely important when engineering algal cells for liquid biofuel production. However, conventional methods for such determination and quantification are not only des...

    Liang-da Chiu, Shih-Hsin Ho, Rintaro Shimada, Nan-Qi Ren in Biotechnology for Biofuels (2017)

  6. Article

    Open Access

    Lignocellulosic saccharification by a newly isolated bacterium, Ruminiclostridium thermocellum M3 and cellular cellulase activities for high ratio of glucose to cellobiose

    Lignocellulosic biomass is one of earth’s most abundant resources, and it has great potential for biofuel production because it is renewable and has carbon-neutral characteristics. Lignocellulose is mainly com...

    Tao Sheng, Lei Zhao, Ling-Fang Gao, Wen-Zong Liu, Min-Hua Cui in Biotechnology for Biofuels (2016)

  7. Article

    Open Access

    Combined cell-surface display- and secretion-based strategies for production of cellulosic ethanol with Saccharomyces cerevisiae

    Engineering Saccharomyces cerevisiae to produce heterologous cellulases is considered as a promising strategy for production of bioethanol from lignocellulose. The production of cellulase is usually pursued by on...

    Zhuo Liu, Kentaro Inokuma, Shih-Hsin Ho, Riaan den Haan in Biotechnology for Biofuels (2015)

  8. Article

    Open Access

    Dynamic metabolic profiling of the marine microalga Chlamydomonas sp. JSC4 and enhancing its oil production by optimizing light intensity

    Marine microalgae are among the most promising lipid sources for biodiesel production because they can be grown on nonarable land without the use of potable water. Marine microalgae also harvest solar energy e...

    Shih-Hsin Ho, Akihito Nakanishi, **aoting Ye, Jo-Shu Chang in Biotechnology for Biofuels (2015)

  9. Article

    Open Access

    Optimizing biodiesel production in marine Chlamydomonassp. JSC4 through metabolic profiling and an innovative salinity-gradient strategy

    Biodiesel production from marine microalgae has received much attention as microalgae can be cultivated on non-arable land without the use of potable water, and with the additional benefits of mitigating CO2 emis...

    Shih-Hsin Ho, Akihito Nakanishi, **aoting Ye, Jo-Shu Chang in Biotechnology for Biofuels (2014)

  10. Article

    Open Access

    Glycogen production for biofuels by the euryhaline cyanobacteria Synechococcus sp. strain PCC 7002 from an oceanic environment

    Oxygenic photosynthetic microorganisms such as cyanobacteria and microalgae have attracted attention as an alternative carbon source for the next generation of biofuels. Glycogen abundantly accumulated in cyan...

    Shimpei Aikawa, Atsumi Nishida, Shih-Hsin Ho, Jo-Shu Chang in Biotechnology for Biofuels (2014)