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16 Result(s)

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  1. No Access

    Protocol

    Escherichia coli-Based Expression and In Vitro Activity Assay of 1-Aminocyclopropane-1-Carboxylate (ACC) Synthase and ACC Oxidase

    1-Aminocyclopropane-1-carboxylate (ACC) synthase and ACC oxidase are key enzymes in the ethylene biosynthetic pathway in plant tissues, and in vitro assay of their activities is indispensable for analysis, esp...

    Shigeru Satoh, Yusuke Kosugi in Ethylene Signaling (2017)

  2. No Access

    Article

    Expression of a rice glutaredoxin in aleurone layers of develo** and mature seeds: subcellular localization and possible functions in antioxidant defense

    A rice glutaredoxin isoform (OsGrxC2;2) with antioxidant capacity is expressed abundantly in seed tissues and is localized to storage vacuoles in aleurone layers in develo** and mature seeds.

    Shigeto Morita, Yuki Yamashita, Masayoshi Fujiki, Rie Todaka, Yuri Nishikawa… in Planta (2015)

  3. No Access

    Article

    2-Aminooxyisobutyric acid inhibits the in vitro activities of both 1-Aminocyclopropane-1-carboxylate (ACC) synthase and ACC oxidase in ethylene biosynthetic pathway and prolongs vase life of cut carnation flowers

    2-Aminooxyisobutyric acid (AOIB) has a partial structure of aminooxyacetic acid (AOA) in its whole structure, and resembles 2-aminoisobutyric acid (AIB) in their tetrahedral structures. Both AOA and AIB are in...

    Yusuke Kosugi, Asami Matsuoka, Akane Higashi, Noriko Toyohara… in Journal of Plant Biology (2014)

  4. No Access

    Article

    RNAi-mediated suppression of endogenous storage proteins leads to a change in localization of overexpressed cholera toxin B-subunit and the allergen protein RAG2 in rice seeds

    RNAi-mediated suppression of the endogenous storage proteins in MucoRice-CTB-RNAi seeds affects not only the levels of overexpressed CTB and RAG2 allergen, but also the localization of CTB and ...

    Shiho Kurokawa, Masaharu Kuroda, Mio Mejima, Rika Nakamura… in Plant Cell Reports (2014)

  5. No Access

    Article

    Accumulation of rice prolamin–GFP fusion proteins induces ER-derived protein bodies in transgenic rice calli

    We showed that rice prolamin polypeptides formed ER-derived PBs in transgenic rice calli, and that this heterologous transgene expression system is suitable for studying the mechanism of rice P...

    Takanari Shigemitsu, Takehiro Masumura, Shigeto Morita, Shigeru Satoh in Plant Cell Reports (2013)

  6. No Access

    Article

    Early flowering and increased expression of a FLOWERING LOCUS T-like gene in chrysanthemum transformed with a mutated ethylene receptor gene mDG-ERS1(etr1-4)

    Ethylene has an inhibitory effect on flowering in a short-day (SD) plant chrysanthemum (Chrysanthemum morifolium Ramat.). In this study, we used a hexaploid chrysanthemum ‘Sei-Marine’ and found that its transgeni...

    Shigeto Morita, Yuino Murakoshi, Ai Hojo, Keiko Chisaka… in Journal of Plant Biology (2012)

  7. No Access

    Article

    Production of human growth hormone in transgenic rice seeds: co-introduction of RNA interference cassette for suppressing the gene expression of endogenous storage proteins

    Rice seeds are potentially useful hosts for the production of pharmaceutical proteins. However, low yields of recombinant proteins have been observed in many cases because recombinant proteins compete with end...

    Takanari Shigemitsu, Shinji Ozaki, Yuhi Saito, Masaharu Kuroda… in Plant Cell Reports (2012)

  8. No Access

    Article

    Analysis of genomic DNA of DcACS1, a 1-aminocyclopropane-1-carboxylate synthase gene, expressed in senescing petals of carnation (Dianthus caryophyllus) and its orthologous genes in D. superbus var. longicalycinus

    Carnation (Dianthus caryophyllus) flowers exhibit climacteric ethylene production followed by petal wilting, a senescence symptom. DcACS1, which encodes 1-aminocyclopropane-1-carboxylate synthase (ACS), is a gene...

    Taro Harada, Yuino Murakoshi, Yuka Torii, Koji Tanase… in Plant Cell Reports (2011)

  9. No Access

    Article

    Subcellular Localization and Changes in mRNA Abundance of CEBP, a Nuclear-Encoded Chloroplast Protein, During Flower Development and Senescence

    CEBP, a nuclear-encoded chloroplast protein, has been previously cloned as a putative transcription factor involved in ethylene signaling in carnation development and senescence. In order to more clearly defin...

    Mihaela Iordachescu, Heidi Bowman, Kentaro Sasaki, Ryozo Imai… in Journal of Plant Biology (2009)

  10. No Access

    Article

    Suppressed Leaf Senescence in Chrysanthemum Transformed with a Mutated Ethylene Receptor GenemDG-ERS1(etr1-4)

    Previously, Narumi et al. (2005) generated chrysanthemum plants transformed with a mutated ethylene receptor gene (mDG-ERS1(etr1-4<), and showed that thein vitro plantlets of the transformants grown aseptically i...

    Shigeru Satoh, Masanobu Watanabe, Keiko Chisaka, Takako Narumi in Journal of Plant Biology (2008)

  11. No Access

    Article

    Anoxia-enhanced expression of genes isolated by suppression subtractive hybridization from pondweed (Potamogeton distinctus A. Benn.) turions

    Pondweed (Potamogeton distinctus A. Benn.), a monocot aquatic plant species, has turions, which are overwintering buds forming underground as an asexual reproductive organ. Turions not only survive for more than ...

    Taro Harada, Shigeru Satoh, Toshihito Yoshioka, Kimiharu Ishizawa in Planta (2007)

  12. No Access

    Article

    Transformation of carnation with genes related to ethylene production and perception: towards generation of potted carnations with a longer display time

    Potted carnation (Dianthus caryophyllus L. cv. Lillipot) plants were transformed with cDNAs for carnation 1-aminocyclopropane-1-carboxylate (ACC) synthase (DC-ACS1, s/aACS transgenes) or ACC oxidase (DC-ACO1, s/a...

    Tomoyuki Kinouchi, Reiko Endo, Atsushi Yamashita… in Plant Cell, Tissue and Organ Culture (2006)

  13. No Access

    Article

    Characteristics of the inhibitory action of 1,1-dimethyl-4-(phenylsulfonyl)semicarbazide (DPSS) on ethylene production in carnation (Dianthus caryophyllus L.) flowers

    1,1-Dimethyl-4-(phenylsulfonyl)semicarbazide (DPSS)inhibited ethylene productionin carnation flowers during natural senescence, butdid not inhibit the ethyleneproduction induced by exogenous ethylene in carnat...

    Tomoko Onoue, Mariko Mikami, Toshihto Yoshioka… in Plant Growth Regulation (2000)

  14. No Access

    Article

    Induction by ozone of ethylene production and an ACC oxidase cDNA in rice (Oryza sativa L.) leaves

    Exposure to ozone at 1 µl l−1 for 6 h induced ethylene production in rice (Oryza sativa L. cv. Hitomebore) leaves. The stimulation of ethylene production was detectable 2 h after the start of the exposure to ozon...

    Takehiro Ohki, Hideki Matsui, Atsushi Nagasaka… in Plant Growth Regulation (1999)

  15. No Access

    Article

    1,1-Dimethyl-4-(phenylsulfonyl)semicarbazide (DPSS) does not inhibit the in vitro activities of 1-aminocyclopropane-1-carboxylate (ACC) oxidase and ACC synthase obtained from senescing carnation Dianthus caryophyllus L.) petals

    The effects of 1,1-dimethyl-4-(phenylsulfonyl)semicarbazide (DPSS) on the in vitro activities of 1-aminocyclopropane-1-carboxylate (ACC) oxidase and ACC synthase isolated from senescing carnation petals were i...

    Shigeru Satoh, Naoko Oyamada, Toshihito Yoshioka, Naoki Midoh in Plant Growth Regulation (1997)

  16. No Access

    Article

    D-Amino-acid-stimulated ethylene production in seed tissues

    Ethylene production by axial and cotyledonary tissues excised from Xanthium pennsylvanicum Wallr. seeds was markedly (up to 5-fold) stimulated by the D-isomers of phenylalanine, valine, leucine, threonine, methio...

    Shigeru Satoh, Yohji Esashi in Planta (1980)