10 Result(s)
within
Sergio Martinez Cuesta
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Article
Open AccessA TRIM21-based bioPROTAC highlights the therapeutic benefit of HuR degradation
Human antigen R (HuR) is a ubiquitously expressed RNA-binding protein, which functions as an RNA regulator. Overexpression of HuR correlates with high grade tumours and poor patient prognosis, implicating it a...
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Open AccessGenome surveillance by HUSH-mediated silencing of intronless mobile elements
All life forms defend their genome against DNA invasion. Eukaryotic cells recognize incoming DNA and limit its transcription through repressive chromatin modifications. The human silencing hub (HUSH) complex t...
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Article
Open AccessRNA G-quadruplex structures control ribosomal protein production
Four-stranded G-quadruplex (G4) structures form from guanine-rich tracts, but the extent of their formation in cellular RNA and details of their role in RNA biology remain poorly defined. Herein, we first deli...
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Open AccessChemical profiling of DNA G-quadruplex-interacting proteins in live cells
DNA–protein interactions regulate critical biological processes. Identifying proteins that bind to specific, functional genomic loci is essential to understand the underlying regulatory mechanisms on a molecul...
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Open AccessG-quadruplexes are transcription factor binding hubs in human chromatin
The binding of transcription factors (TF) to genomic targets is critical in the regulation of gene expression. Short, double-stranded DNA sequence motifs are routinely implicated in TF recruitment, but many qu...
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Article
Sequencing abasic sites in DNA at single-nucleotide resolution
In DNA, the loss of a nucleobase by hydrolysis generates an abasic site. Formed as a result of DNA damage, as well as a key intermediate during the base excision repair pathway, abasic sites are frequent DNA l...
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Article
5-Formylcytosine organizes nucleosomes and forms Schiff base interactions with histones in mouse embryonic stem cells
Nucleosomes are the basic unit of chromatin that help the packaging of genetic material while controlling access to the genetic information. The underlying DNA sequence, together with transcription-associated ...
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DNA G-quadruplex structures mold the DNA methylome
Control of DNA methylation level is critical for gene regulation, and the factors that govern hypomethylation at CpG islands (CGIs) are still being uncovered. Here, we provide evidence that G-quadruplex (G4) D...
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Article
Open AccessBase resolution maps reveal the importance of 5-hydroxymethylcytosine in a human glioblastoma
Aberrant genetic and epigenetic variations drive malignant transformation and are hallmarks of cancer. Using PCR-free sample preparation we achieved the first in-depth whole genome (hydroxyl)-methylcytosine, s...
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Article
EC-BLAST: a tool to automatically search and compare enzyme reactions
EC-BLAST is a Web-based tool allowing quantitative similarity searches between enzymes at the levels of bond-change, reaction-center or reaction-structure similarity. The tool may help improve the annotation o...
