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Open AccessRecovering true FRET efficiencies from smFRET investigations requires triplet state mitigation
Single-molecule fluorescence resonance energy transfer (smFRET) methods employed to quantify time-dependent compositional and conformational changes within biomolecules require elevated illumination intensitie...
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Article
Open AccessGeometric alignment of aminoacyl-tRNA relative to catalytic centers of the ribosome underpins accurate mRNA decoding
Accurate protein synthesis is determined by the two-subunit ribosome’s capacity to selectively incorporate cognate aminoacyl-tRNA for each mRNA codon. The molecular basis of tRNA selection accuracy, and how fi...
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Open AccessAuthor Correction: CFTR function, pathology and pharmacology at single-molecule resolution
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Article
Open AccessmRNA decoding in human is kinetically and structurally distinct from bacteria
In all species, ribosomes synthesize proteins by faithfully decoding messenger RNA (mRNA) nucleotide sequences using aminoacyl-tRNA substrates. Current knowledge of the decoding mechanism derives principally f...
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Open AccessCFTR function, pathology and pharmacology at single-molecule resolution
The cystic fibrosis transmembrane conductance regulator (CFTR) is an anion channel that regulates salt and fluid homeostasis across epithelial membranes1. Alterations in CFTR cause cystic fibrosis, a fatal diseas...
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Article
Synthesis and single-molecule imaging reveal stereospecific enhancement of binding kinetics by the antitumour eEF1A antagonist SR-A3
Ternatin-family cyclic peptides inhibit protein synthesis by targeting the eukaryotic elongation factor-1α. A potentially related cytotoxic natural product (‘A3’) was isolated from Aspergillus, but only 4 of its ...
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Open AccessThe chemotherapeutic CX-5461 primarily targets TOP2B and exhibits selective activity in high-risk neuroblastoma
Survival in high-risk pediatric neuroblastoma has remained around 50% for the last 20 years, with immunotherapies and targeted therapies having had minimal impact. Here, we identify the small molecule CX-5461 ...
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Open AccessStructural basis of early translocation events on the ribosome
Peptide-chain elongation during protein synthesis entails sequential aminoacyl-tRNA selection and translocation reactions that proceed rapidly (2–20 per second) and with a low error rate (around 10−3 to 10−5 at e...
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Article
Single-molecule FRET imaging of GPCR dimers in living cells
Class C G protein-coupled receptors (GPCRs) are known to form stable homodimers or heterodimers critical for function, but the oligomeric status of class A and B receptors, which constitute >90% of all GPCRs, ...
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Article
Subnanometer structures of HIV-1 envelope trimers on aldrithiol-2-inactivated virus particles
The HIV-1 envelope glycoprotein (Env) trimer, composed of gp120 and gp41 subunits, mediates viral entry into cells. Recombinant Env trimers have been studied structurally, but characterization of Env embedded ...
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Author Correction: Quantifying secondary transport at single-molecule resolution
An Amendment to this paper has been published and can be accessed via a link at the top of the paper.
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Open AccessX-ray structure of LeuT in an inward-facing occluded conformation reveals mechanism of substrate release
Neurotransmitter:sodium symporters (NSS) are conserved from bacteria to man and serve as targets for drugs, including antidepressants and psychostimulants. Here we report the X-ray structure of the prokaryotic...
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Open AccessDisruption of the HIV-1 Envelope allosteric network blocks CD4-induced rearrangements
The trimeric HIV-1 Envelope protein (Env) mediates viral-host cell fusion via a network of conformational transitions, with allosteric elements in each protomer orchestrating host receptor-induced exposure of ...
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Article
Cargo adaptors regulate step** and force generation of mammalian dynein–dynactin
Cytoplasmic dynein is an ATP-driven motor that transports intracellular cargos along microtubules. Dynein adopts an inactive conformation when not attached to a cargo, and motility is activated when dynein ass...
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Article
Quantifying secondary transport at single-molecule resolution
Secondary active transporters, which are vital for a multitude of physiological processes, use the energy of electrochemical ion gradients to power substrate transport across cell membranes1,2. Efforts to investi...
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Open AccessCorrection to: Implications of sequence variation on the evolution of rRNA
The article Implications of sequence variation on the evolution of rRNA, written by Matthew M. Parks, Chad M. Kurylo, Jake E. Batchelder, C. Theresa Vincent and Scott C. Blanchard, was originally published ele...
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Open AccessRibosome biogenesis during cell cycle arrest fuels EMT in development and disease
Ribosome biogenesis is a canonical hallmark of cell growth and proliferation. Here we show that execution of Epithelial-to-Mesenchymal Transition (EMT), a migratory cellular program associated with development...
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Article
ADAM10 controls the differentiation of the coronary arterial endothelium
The coronary vasculature is crucial for normal heart function, yet much remains to be learned about its development, especially the maturation of coronary arterial endothelium. Here, we show that endothelial i...
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Associating HIV-1 envelope glycoprotein structures with states on the virus observed by smFRET
The HIV-1 envelope glycoprotein (Env) trimer mediates cell entry and is conformationally dynamic1–8. Imaging by single-molecule fluorescence resonance energy transfer (smFRET) has revealed that, on the surface of...
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Article
Open AccessImplications of sequence variation on the evolution of rRNA
The evolution of the multi-copy family of ribosomal RNA (rRNA) genes is unique in regard to its genetics and genome evolution. Paradoxically, rRNA genes are highly homogenized within and between individuals, y...
