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Article
Optimized protocol for isolation and transient expression of placenta-originated protoplast in pepper (Capsicum annuum L.)
Pepper (Capsicum annuum L.) is one of the most widely cultivated species and is highly valued for its pungency. The pungency of pepper is primarily attributed to a group of chemical compounds known as capsaicinoi...
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Article
Development of a general protoplast-mediated regeneration protocol for Brassica: cabbage and cauliflower as examples
The genus Brassica includes vegetables such as broccoli, cabbage, cauliflower, kale, and kohlrabi, which are economically important because they contain beneficial compounds, such as glucosinolates. Clustered reg...
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Article
Mutation in the GA3ox gene governs short-internode characteristic in a korean cucumber inbred line
Here, we identified a new natural mutant, si-2, from a Korean cucumber inbred line. The genes responsible for dwarf (DW) and/or short internode (SI) in cucurbits were selected and used to identify the gene(s) con...
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Article
Development of glucoraphanin-rich broccoli (Brassica oleracea var. italica) by CRISPR/Cas9-mediated DNA-free BolMYB28 editing
Broccoli, a Brassica crop, is an important vegetative crop because of its nutritional components and beneficial phytochemicals. Glucoraphanin (GR), a major glucosinolate (GSL) in broccoli, is converted by hydroly...
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Article
QTL-seq analysis of powdery mildew resistance in a Korean cucumber inbred line
QTL map** and RT-PCR analyses identified the CsGy5G015660 as a strong powdery mildew resistance candidate gene and natural variation of CsGy5G015660 allele was observed using 115 core germplasm.
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Article
Open AccessCritical enzymes for biosynthesis of cucurbitacin derivatives in watermelon and their biological significance
Various cucurbitacins have been isolated, and their structures have been elucidated. Owing to their economic potential and importance as active pharmacological compounds, their cytotoxicity in various cancer c...
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Article
Examination of glucoraphanin content in broccoli seedlings over growth and the impact of hormones and sulfur-containing compounds
In this study, we found that the glucoraphanin (GR) content in broccoli seedlings decreased before first foliage leaf emergence. Methyl jasmonate (MeJA) treatment significantly increased the GR content in broc...
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Article
Impact of Agrobacterium-infiltration and transient overexpression of BroMYB28 on glucoraphanin biosynthesis in broccoli leaves
To date studies revealed that MYB28 intensively involved biosynthesis of GSLs, there is no direct evidence of MYB28 in glucoraphanin (GR) biosynthesis of broccoli. Furthermore, Agrobacterium (Agro)-infiltration a...
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Article
Two types of mutations in the HEUKCHEEM gene functioning in cucumber spine color development can be used as signatures for cucumber domestication
The HEUKCHEEM gene plays an important role in spine color formation. A white spine occurs due to two mutations in HEUKCHEEM and is closely related to the regional distribution of these mutants.
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Article
Identification of quantitative trait loci governing subgynoecy in cucumber
QTL-seq analysis identified three major QTLs conferring subgynoecy in cucumbers. Furthermore, sequence and expression analyses predicted candidate genes controlling subgynoecy.
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Article
Radiation-Induced Breeding in Camelina Sativa
The ionizing radiation methods, including X-rays, gamma rays, and neutrons to create variation is well established and used in major crops. Many seed-propagated crops with homozygous parents such as barley, ri...
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Article
Transgenic Arabidopsis plants expressing CsBCATs affect seed germination under abiotic stress conditions
We investigated the responses of transgenic Arabidopsis plants that express cucumber branched-chain aminotransferases (CsBCATs) under abiotic or biotic stresses. The expression of CsBCAT3 and CsBCAT7 was dramatic...
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Article
The overexpression of cucumber (Cucumis sativus L.) genes that encode the branched-chain amino acid transferase modulate flowering time in Arabidopsis thaliana
The overexpression of CsBCATs promotes flowering in Arabidopsis by regulating the expression of flowering time genes.
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Article
Profiling cucurbitacins from diverse watermelons (Citrullus spp.)
Cucurbitacins are a group of tetracyclic triterpenes produced by members of the Cucurbitaceae family that are known for their bitter taste, and have both positive and negative attributes based on their use. The a...
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Article
Genome-wide identification and description of MLO family genes in pumpkin (Cucurbita maxima Duch.)
The Mildew resistance locus o (Mlo) is a plant-specific gene family that encodes a protein with seven-transmembrane (TM) domains that play an important role in plant resistance to powdery mildew (PM). PM caused b...
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Article
RNA expression, protein activity, and interactions in the ACC synthase gene family in cucumber (Cucumis sativus L.)
Ethylene is an important regulator of developmental and stress responses in plants, and 1-aminocyclopropane-1-carboxylic acid synthase (ACS) proteins catalyze one of the rate-limiting steps in endogenous ethyl...
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Article
Characterization of twenty Camelina spp. accessions using single nucleotide polymorphism genoty**
Sequencing the complete genome of Camelina sativa will facilitate studies to improve this oilseed crop. We analysed 20 accessions of Camelina spp. using genoty**-by-sequence technology. After stringent screenin...
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Article
Indigenous parasitoids as effective natural enemies of Phyllocnistis citrella (Lepidoptera: Gracillariidae) in Korea
This study is aimed to investigate the occurrence of Phyllocnistis citrella Stainton (citrus leafminer; CLM), which has recently caused increasing damage to citrus farms in southern South Korea, and its natural e...
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Article
QTL map** for downy mildew resistance in cucumber via bulked segregant analysis using next-generation sequencing and conventional methods
QTL map** using NGS-assisted BSA was successfully applied to an F 2 population for downy mildew resistance in cucum...
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Article
Heterologous expression of an RNA-binding protein affects flowering time as well as other developmental processes in Solanaceae
Flowering time in members of the Solanaceae plant family, such as pepper (Capsicum spp.) and tomato (Solanum lycopersicum), is an important agronomic trait for controlling shoot architecture and improving yield. ...
