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Article
Characterization of a novel cold-active esterase isolated from swamp sediment metagenome
A functional screen of a metagenomic library from “Upo” swamp sediment in Korea identified a gene EstL28, the product of which displayed lipolytic properties on a tributyrin-supplemented medium. The EstL28 sequen...
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Article
Burkholderia denitrificans sp. nov., isolated from the soil of Dokdo Island, Korea
A novel, Gram-negative, bacterial strain KIS30-44T was identified from wet forest soil collected on the Korean island of Dokdo. Growth of the strain was observed at 15–30°C, pH 5–9, 0–3% NaCl, and 950 mM KNO3. KI...
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Article
Expression of the Human Tissue-Plasminogen Activator in Hairy Roots of Oriental Melon (Cucumis melo)
The human tissue plasminogen activator (t-PA) is a thrombolytic protein that plays a key role in fibrinolysis by converting the plasminogen into plasmin which degrades fibrin clots in blood vessels. The t-PA gene...
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Article
Characterization of Capsicum annuum Recombinant α- and β-Tubulin
There are several conditions which might modulate polymerization to produce polymers having normal lattice structure. In the absence of 1 mM MgCl2 the assembly was reduced by 36% in Capsicum annuum tubulin (CAnm ...
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Article
α- and β-tubulin from Phytophthora capsici KACC 40483: molecular cloning, biochemical characterization, and antimicrotubule screening
Internal fragments of α- and β-tubulin genes were generated using reverse transcription polymerase chain reaction (RT-PCR), and the termini were isolated using 5′- and 3′-rapid amplification of cDNA ends. Phytoph...
