6 Result(s)
within
Samuel Wagner
Microbiology
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Protocol
Blue Native PAGE Analysis of Bacterial Secretion Complexes
Bacterial protein secretion systems serve to translocate substrate proteins across up to three biological membranes, a task accomplished by hydrophobic, membrane-spanning macromolecular complexes. The overexpr...
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Protocol
Analysis of SPI-1 Dependent Type III Secretion and Injection Using a NanoLuc Luciferase-Based Assay
Studies of bacterial protein secretion have relied on a variety of reporters that allow the tracking of secreted proteins. However, the lack of truly quantitative and highly sensitive reporters has hindered, i...
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Article
Journal Club
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Article
Export von Membranproteinen durch bakterielle Injektionsnadeln
Many pathogenic bacteria use type III secretion systems (T3SS) to deliver effector proteins directly into the host cell cytoplasm. Substrate proteins of these systems are mostly soluble, however, a number of h...
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Protocol
Blue Native PAGE Analysis of Bacterial Secretion Complexes
Bacterial protein secretion systems serve to translocate substrate proteins across up to three biological membranes, a task accomplished by hydrophobic, membrane-spanning macromolecular complexes. The overexpr...
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Article
Open AccessA structurally informed autotransporter platform for efficient heterologous protein secretion and display
The self-sufficient autotransporter (AT) pathway, ubiquitous in Gram-negative bacteria, combines a relatively simple protein secretion mechanism with a high transport capacity. ATs consist of a secreted passen...
