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Blue Native PAGE Analysis of Bacterial Secretion Complexes
Bacterial protein secretion systems serve to translocate substrate proteins across up to three biological membranes, a task accomplished by hydrophobic, membrane-spanning macromolecular complexes. The overexpr...
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Article
Spatiotemporal variation of behavior and repeatability in a long-lived turtle
Animal personalities represent repeatable behavioral differences exhibited by individuals in a population. Variation in personality traits across individuals is an increasingly recognized mechanism for species...
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Article
Open AccessAnalysis of a Real-World Progression Variable and Related Endpoints for Patients with Five Different Cancer Types
We previously demonstrated that real-world progression (rwP) can be ascertained from unstructured electronic health record (EHR)-derived documents using a novel abstraction approach for patients with advanced ...
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Protocol
Analysis of SPI-1 Dependent Type III Secretion and Injection Using a NanoLuc Luciferase-Based Assay
Studies of bacterial protein secretion have relied on a variety of reporters that allow the tracking of secreted proteins. However, the lack of truly quantitative and highly sensitive reporters has hindered, i...
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Chapter
A Lutheran Reflection on Lived Ecumenism
Drawing from twenty years of ecumenical experience, the author considers positive impacts and lessons learned as a Lutheran living and working alongside Catholic peers, colleagues, and mentors. Beginning with ...
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Article
Open AccessThe substrate specificity switch FlhB assembles onto the export gate to regulate type three secretion
Protein secretion through type-three secretion systems (T3SS) is critical for motility and virulence of many bacteria. Proteins are transported through an export gate containing three proteins (FliPQR in flage...
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Chapter
A Unified Nomenclature for Injectisome-Type Type III Secretion Systems
The independent naming of components of injectisome-type type III secretion systems in different bacterial species has resulted in considerable confusion, impeding accessibility of the literature and hindering...
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Journal Club
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Article
Open AccessRevealing the mechanisms of membrane protein export by virulence-associated bacterial secretion systems
Many bacteria export effector proteins fulfilling their function in membranes of a eukaryotic host. These effector membrane proteins appear to contain signals for two incompatible bacterial secretion pathways ...
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Article
Author Correction: Structure of the core of the type III secretion system export apparatus
In the version of this article initially published, the PDB code associated with the study was given as 6F2E but should have been 6F2D in Table 1 and the data availability statement. The error has been correct...
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Article
Structure of the core of the type III secretion system export apparatus
Export of proteins through type III secretion systems is critical for motility and virulence of many major bacterial pathogens. Three putative integral membrane proteins (FliP, FliQ, FliR) are suggested to for...
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Article
Export von Membranproteinen durch bakterielle Injektionsnadeln
Many pathogenic bacteria use type III secretion systems (T3SS) to deliver effector proteins directly into the host cell cytoplasm. Substrate proteins of these systems are mostly soluble, however, a number of h...
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Article
Open AccessIsolation and characterization of the E. coli membrane protein production strain Mutant56(DE3)
Membrane protein production is usually toxic to E. coli. However, using genetic screens strains can be isolated in which the toxicity of membrane protein production is reduced, thereby improving production yields...
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Protocol
Blue Native PAGE Analysis of Bacterial Secretion Complexes
Bacterial protein secretion systems serve to translocate substrate proteins across up to three biological membranes, a task accomplished by hydrophobic, membrane-spanning macromolecular complexes. The overexpr...
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Article
Open AccessEnhancing quantum sensing sensitivity by a quantum memory
In quantum sensing, precision is typically limited by the maximum time interval over which phase can be accumulated. Memories have been used to enhance this time interval beyond the coherence lifetime and thus...
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Article
Open AccessA structurally informed autotransporter platform for efficient heterologous protein secretion and display
The self-sufficient autotransporter (AT) pathway, ubiquitous in Gram-negative bacteria, combines a relatively simple protein secretion mechanism with a high transport capacity. ATs consist of a secreted passen...
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Article
Open AccessHealth related quality of life outcomes for unresectable stage III or IV melanoma patients receiving ipilimumab treatment
In an international, randomized Phase III trial ipilimumab demonstrated a significant overall survival benefit in previously treated advanced melanoma patients. This report summarizes health-related quality of...
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Protocol
Comparative Analysis of Cytoplasmic Membrane Proteomes of Escherichia coli Using 2D Blue Native/SDS-PAGE
Two-dimensional blue native (2D BN)/SDS-PAGE is the method of choice for the global analysis of the subunits of complexes in membrane proteomes. In the 1st dimension complexes are separated by BN-PAGE, and in ...
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Book
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Chapter
Project Selection, Commercial Risks, and the Allocation to Small Business of Federal Research and Development Contracts
The previous chapter was concerned with the nature, organization, and technical outcome of industrial research and development. In this chapter, we continue to focus on industrial research and development, but...
