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  1. No Access

    Article

    High Throughput Screening in Drug Discovery: Problems and Solutions

    World-wide introduction of high throughput screening (HTS) methods in drug discovery research did not result in the increased number of novel medications on the market. We discuss novel trends in drug discover...

    D. M. Hushpulian, I. N. Gaisina, S. V. Nikulin… in Moscow University Chemistry Bulletin (2024)

  2. No Access

    Article

    Synthesis of the Diamond‒SiO2 Composites with the Properties of Photonic Crystals in the Visible Spectral Range

    The chemical vapor deposition synthesis of periodic structures in the form of single-crystal diamond–SiO2 nanosphere composites, which exhibit the properties of photonic crystals in the visible spectral range, ar...

    D. N. Sovyk, K. A. Odintsov, A. P. Bolshakov, S. A. Dyakov, S. S. Savin… in Doklady Physics (2023)

  3. No Access

    Article

    Engineering the N-Terminal Sequence of Glycine max Soybean Formate Dehydrogenase

    NAD(P)+-dependent formate dehydrogenase (FDH, EC 1.2.1.2.) catalyzes the oxidation of formate ion with the coupled reduction of NAD(P)+ to NAD(P)H. Previously, in our laboratory, a genetic construct was obtained ...

    L. A. Shaposhnikov, S. S. Savin, D. L. Atroshenko… in Moscow University Chemistry Bulletin (2023)

  4. No Access

    Article

    Formate Dehydrogenase: From NAD(P)H Regeneration to Targeting Pathogen Biofilms, Composing Highly Efficient Hybrid Biocatalysts and Atmospheric CO2 Fixation

    NAD(P)+-dependent formate dehydrogenase (EC 1.2.1.2, FDH) catalyzes the simplest reaction from chemical and biological points of view, oxidation of formate-ion to carbon dioxide coupled to NAD(P)+ reduction to yi...

    V. I. Tishkov, A. A. Pometun, S. S. Savin in Moscow University Chemistry Bulletin (2023)

  5. No Access

    Article

    Kinetics of Thermoinactivation of D-Amino Acid Oxidase OPADAAO1 from the Ogataea parapolymorpha DL-1 Yeast

    Our earlier annotation of the genome of the yeast Ogataea parapolymorpha DL-1 made it possible to identify five genes of potential D-amino acids oxidases. All opadaao1–opadaao5 genes were cloned and expressed in

    M. K. Koshkina, E. P. Sergeyev, T. A. Fedorov… in Moscow University Chemistry Bulletin (2023)

  6. No Access

    Article

    Highly Stable Mutant Bacterial Formate Dehydrogenase with Improved Catalytic Properties

    NAD+-dependent formate dehydrogenase (FDH, EC 1.2.1.2) from methylotrophic bacterium Pseudomonas sp.101 (PseFDH) has one of the highest thermal stability among all known enzymes of this group. The introduction of...

    A. A. Pometun, A. A. Shirokova, N. P. Galanicheva… in Moscow University Chemistry Bulletin (2023)

  7. No Access

    Article

    Physiological Role of D-amino Acids and Bioanalytical Potential of D-amino Acid Oxidases

    D-amino acid oxidase (DAAO) plays an important role in the functioning of both prokaryotes and eukaryotes. DAAO is increasingly being used in practice, including for the determination of D-amino acids in compl...

    V. I. Tishkov, M. D. Shelomov, A. A. Pometun… in Moscow University Chemistry Bulletin (2023)

  8. No Access

    Article

    Engineering the Active Site of Formate Dehydrogenase from Staphylococcus aureus: Introduction of the Additional Loop and Histigine Residues to the Structure

    NAD+-dependent formate dehydrogenase (EC 1.2.1.2, FDH) from pathogenic bacterium Staphylococcus aureus (SauFDH) differs significantly from other FDHs both in terms of primary structure and catalytic properties. A...

    T. S. Iurchenko, A. A. Loginova, E. P. Sergeev… in Moscow University Chemistry Bulletin (2023)

  9. No Access

    Article

    Effect of the Components of a Buffer Solution on the Catalytic Activity of the NAD+-Dependent Formate Dehydrogenase from the Bacterium Staphylococcus aureus

    NAD+-dependent formate dehydrogenase (FDH, EC 1.2.1.2) from bacterium Staphylococcus aureus (SauFDH) is the most active enzyme among FDHs of this group; however, the high values of KM of the enzyme with NAD+ and ...

    T. S. Iurchenko, S. B. Bolotova, A. A. Loginova… in Moscow University Chemistry Bulletin (2022)

  10. No Access

    Article

    Study of the Mechanism of Coenzyme Specificity of Phenylacetone Monooxygenase from Thermobifida fusca by Site-Directed Mutagenesis

    Phenylacetone monooxygenase from Thermobifida fusca (EC 1.14.13.92, PAMO) belongs to the Baeyer–Villiger family of monooxygenases and catalyzes the oxidation of various aromatic ketones to the corresponding ester...

    P. D. Parshin, U. A. Martysuk, D. L. Atroshenko… in Moscow University Chemistry Bulletin (2022)

  11. No Access

    Article

    Formation of Germanium–Vacancy Color Centers in CVD Diamond

    We study Ge-doped polycrystalline diamond films synthesized, using microwave plasma chemical vapor deposition (CVD) in CH4-H2 base mixtures. We compare two sources of the dopant – gaseous monogermane (GeH4) and s...

    V. S. Sedov, A. K. Martyanov, A. S. Altakhov… in Journal of Russian Laser Research (2022)

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    Article

    Synthesis of Multilayered Diamond Films in Microwave Plasma with Periodic Nitrogen Injections

    The use of polycrystalline diamond in optics (transparent windows) and electronics (heat sinks) usually requires polishing of a synthesized wafer. However, mechanical polishing of diamond faces certain difficu...

    A. K. Martyanov, V. S. Sedov, E. V. Zavedeev, S. S. Savin… in Doklady Physics (2021)

  13. No Access

    Article

    Preparation of Recombinant Formate Dehydrogenase from Thermotolerant Yeast Ogataea parapolymorpha and Crystallization of Its Apo- and Holo- Forms

    NAD+-dependent formate dehydrogenase from thermotolerant yeast Ogataea parapolymorpha DL-1 (OpaFDH, EC 1.2.1.2) with the additional N-terminal Gly residue and its double mutant OpaFDH_AD were overexpressed in E. ...

    A. A. Pometun, K. M. Boyko, S. A. Zubanova… in Moscow University Chemistry Bulletin (2021)

  14. No Access

    Article

    Influence of His6 Sequence on the Properties of Formate Dehydrogenase from Bacterium Pseudomonas sp. 101

    NAD(P)+-dependent formate dehydrogenase (FDH, EC 1.2.1.2.) is actively used in processes of chiral synthesis by oxidoreductases with systems of reduced cofactor regeneration. The efficient use of FDH in such syst...

    A. A. Pometun, P. D. Parshin, N. P. Galanicheva… in Moscow University Chemistry Bulletin (2020)

  15. No Access

    Article

    Highly-Active Recombinant Formate Dehydrogenase from Pathogenic Bacterium Staphylococcus aureus: Preparation and Crystallization

    NAD+-dependent formate dehydrogenase from Staphylococcus aureus (SauFDH) is one of the key enzymes responsible for the survival of this pathogen in the form of biofilms. 3D structure of the enzyme might be helpfu...

    A. A. Pometun, K. M. Boyko, T. S. Yurchenko, A. Yu. Nikolaeva… in Biochemistry (Moscow) (2020)

  16. No Access

    Article

    Effect of His6-tag Position on the Expression and Properties of Phenylacetone Monooxygenase from Thermobifida fusca

    Phenylacetone monooxygenase (EC 1.14.13.92, PAMO) catalyzes oxidation of ketones with molecular oxygen and NADPH with the formation of esters. PAMO is a promising enzyme for biotechnological processes. In this...

    P. D. Parshin, A. A. Pometun, U. A. Martysuk, S. Yu. Kleymenov… in Biochemistry (Moscow) (2020)

  17. No Access

    Article

    Laser-Assisted Formation of High-Quality Polycrystalline Diamond Membranes

    A polycrystalline diamond film is grown on a 2 inch Si substrate using a microwave-plasma chemicalvapor-deposition technique. The high quality of the diamond films is confirmed by Raman spectra. A multiple-ste...

    V. S. Sedov, A. A. Voronin, M. S. Komlenok… in Journal of Russian Laser Research (2020)

  18. No Access

    Article

    Double-Lattice Magnetoplasmonic Structures Based on BIG and Perforated Gold Films

    A method of manufacturing double-lattice magnetoplasmonic crystals with the structure (Au/BIG)2, in which the plasmon gold lattices are displaced relative to each other by half a period, has been presented. Gold ...

    P. N. Naydenov, A. L. Chekhov, O. L. Golikova, A. V. Bespalov… in Physics of the Solid State (2019)

  19. No Access

    Article

    Determination of the Kinetic Parameters of a Wild-Type D-Amino Acid Oxidase from Yeast and Its Mutant Forms in a Reaction of Cephalosporin C Oxidation

    The oxidation of cephalosporin C (CPC) by D-amino acid oxidase is the first stage in the biocatalytic industrial process of the production of 7-aminocephalosporanic acid, the initial synthon for the production...

    D. L. Atroshenko, A. A. Pometun, S. S. Savin… in Moscow University Chemistry Bulletin (2019)

  20. No Access

    Article

    Effect of Medium pH And Ion Strength on the Thermal Stability of Plant Formate Dehydrogenases

    The effect of medium pH and ion strength on the thermal stability of formate dehydrogenases (EC 1.2.1.2, FDH) from model plant Arabidopsis thaliana (AthFDH) and soybean Glycine max (SoyFDH) are studied. The depen...

    A. A. Pometun, N. S. Voinova, E. V. Pometun… in Moscow University Chemistry Bulletin (2018)

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