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Article
Surgical treatment for pyogenic vertebral osteomyelitis using iodine-supported spinal instruments: initial case series of 14 patients
Reports have detailed the increasing use of spinal instrumentation in the treatment of pyogenic vertebral osteomyelitis, with the aims of achieving a lower pseudoarthrosis rate and restoring spinal alignment. ...
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Article
The quantity and diversity of infectious viruses in various tissues of SHIV-infected monkeys at the early and AIDS stages
To detect the major sites of viral replication in immunodeficiency virus-infected individuals, we quantified proviral DNA and infectious viruses using quantitative PCR and a plaque assay, respectively, in vari...
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Article
Haemagglutinin-esterase protein (HE) of murine corona virus: DVIMD (diarrhea virus of infant mice)
The acetylesterase (AE) activity of DVIM (diarrhea virus of infant mice) was assigned to the haemagglutinin-esterase (HE) protein. The substrate specificity was examined using the natural substrate bovine subm...
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Article
Human immunodeficiency virus type 1vif, vpr, andvpu mutants can produce persistently infected cells
A series of human immunodeficiency virus type 1 (HIV-1) mutants invif, vpr, vpu, andnef were constructed from an infectious plasmid (pNL 432) containing the full-length HIV-1 DNA by frameshift mutations. The capa...
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Article
Production of infectious particles from defective human immunodeficiency virus type 1 (HIV-1)-producing cell clones by superinfection with infectious HIV-1
A total of 81 cell clones persistently infected with the LAV-1 or HTLV-IIIB strain of human immunodeficiency virus type 1 (HIV-1) was isolated from cells which were obtained by serial passage of some prolifera...
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Article
Replicating Marek's disease virus (MDV) serotype 2 DNA with inserted MDV serotype 1 DNA sequences in a Marek's disease lymphoblastoid cell line MSB1-41C
We characterized the properties of herpes-type viruses which grew well in a Marek's disease lymphoblastoid cell line, MSB1-41C, inducing cytopathic effect characterized by the formation of syncytial giant cell...
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Article
The budding of defective human immunodeficiency virus type 1 (HIV-1) particles from cell clones persistently infected with HIV-1
Three cell clones producing large numbers of infectious or noninfectious particles of human immunodeficiency virus type 1 (HIV-1), designated M 10/LAV-2, M 16/LAV-3, and MT/LAV-17, were isolated from persisten...
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Article
Cross-reactivity among cowpox, ectromelia and vaccinia viruses with monoclonal antibodies recognizing distinct antigenic determinants in A-type inclusion bodies
Several monoclonal antibodies recognizing distinct antigenic determinants in A-type inclusion bodies (ATIB) induced by cowpox virus (CPV) were obtained to examine the cross-reactivity among various strains of ...
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Article
A comparison of the biological properties of type 2 plaque-producing agent derived from the Cal-1 strain of Marek's disease virus with other related viruses
The serological and biological properties of the type 2 plaque-producing agent (PPA) derived from the Cal-1 strain of Marek's disease virus (MDV) were compared with those of reference strains of the three sero...
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Article
Similarities and dissimilarities in the structure and expression of viral genomes of various virus strains immunologically related to Marek's disease virus
Various strains immunologically related to Marek's disease virus (MDV) have been subdivided into three serotypes: serotype 1, pathogenic strains of MDV and attenuated or apathogenic variants derived from them;...
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Article
Polypeptide analysis with monoclonal antibodies of A type inclusion bodies induced by cowpox virus
The antigen in A type inclusion bodies (ATIB) induced by cowpox virus (CPV) was examined by immunofluorescent staining with monoclonal antibodies (19) and polypeptide analysis. In the immunofluorescence (IF) t...
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Article
Studies on the serological cross-reaction between Marek's disease virus and herpesvirus of turkeys using monoclonal antibodies to major virus-specific polypeptides
A major virus-specific polypeptide antigenically cross-reactive between Marke's disease virus (MDV) and herpesvirus of turkeys (HVT) was characterized by two monoclonal antibodies against MDV. The antigenic de...
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Article
Genetic relatedness of virulent and avirulent strains of Marek's disease virus
Comparative studies using Southern blot hybridization indicated that the avirulent strains of Marek's disease virus (MDV) were probably generated through DNA rearrangement that occurred in the limited area(s) ...
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Article
Cross-reactivity between cowpox and vaccinia viruses with monoclonal antibodies
Hybridoma cell lines producing monoclonal antibodies against cowpox virus (CPV) and vaccinia virus (VV) were established to examine the specific and cross-reactive antigenic determinants of these viruses. Mono...