36 Result(s)

Article

Some key physiological and molecular processes of cold acclimation

Agricultural production worldwide has been severely impacted by cold and freezing stresses. Plant capacity to acclimate to environmental conditions in their immediate vicinity largely control their survival, g...

R. John, N. A. Anjum, S. K. Sopory, N. A. Akram, M. Ashraf in Biologia Plantarum (2016)

Article

Molecular cloning and characterization of salt overly sensitive gene promoter from Brassica juncea (BjSOS2)

Salt Overly Sensitive (SOS) pathway comprising SOS1, SOS2 and SOS3 genes has been recognized as the key mechanism controlling ion homeostasis under salinity stress. SOS2 component of this pathway encodes a serine...

Charanpreet Kaur, Gautam Kumar, Suminder Kaur… in Molecular Biology Reports (2015)

Article

Plant Rabs: Characterization, Functional Diversity, and Role in Stress Tolerance

Rab proteins form the largest family of small guanosine triphosphate (GTP)-binding proteins. The Rab family in plants is divided into eight subfamilies, Rab1, Rab2, Rab5, Rab6, Rab7, Rab8, Rab11, and Rab18. Ph...

Parinita Agarwal, M. K. Reddy, S. K. Sopory… in Plant Molecular Biology Reporter (2009)

Article

Enhancing salt tolerance in a crop plant by overexpression of glyoxalase II

Earlier we have shown the role of glyoxalase overexpression in conferring salinity tolerance in transgenic tobacco. We now demonstrate the feasibility of same in a crop like rice through overproduction of glyo...

Sneh L. Singla-Pareek, Sudesh Kumar Yadav, Ashwani Pareek… in Transgenic Research (2008)

Article

Constitutive overexpression of a stress-inducible small GTP-binding protein PgRab7 from Pennisetum glaucum enhances abiotic stress tolerance in transgenic tobacco

The Rab GTPases are important components of endocytic network in plant cells. Endocytosis participates in the cell’s reaction to extracellular stimuli by desensitizing, down-regulating or recycling receptors a...

Pradeep K. Agarwal, Parinita Agarwal, Parul Jain, Bhavanath Jha… in Plant Cell Reports (2008)

Article

Functional validation of a novel isoform of Na⁺/H⁺ antiporter from Pennisetum glaucum for enhancing salinity tolerance in rice

Salt stress is an environmental factor that severely impairs plant growth and productivity. We have cloned a novel isoform of a vacuolar Na⁺/H⁺ antiporter from Pennisetum glaucum (PgNHX1) that contains 5 transmem...

Dheeraj Verma, Sneh L Singla-Pareek, Divya Rajagopal, M K Reddy… in Journal of Biosciences (2007)

Article

Pennisetum glaucum Na⁺/H⁺ antiporter confers high level of salinity tolerance in transgenic Brassica juncea

Soil salinity is one of the major abiotic stresses affecting plant productivity. We report on the isolation and characterization of a different isoform of vacuolar Na⁺/H⁺ antiporter from Pennisetum glaucum (PgNHX...

Divya Rajagopal, Pradeep Agarwal, Wricha Tyagi, Sneh L. Singla-Pareek… in Molecular Breeding (2007)

Article

Stress-inducible DREB2A transcription factor from Pennisetum glaucum is a phosphoprotein and its phosphorylation negatively regulates its DNA-binding activity

Abiotic stress-mediated gene expression is regulated via different transcription factors of which drought-responsive element-binding (DREB) proteins play an important role. There are two types of DREBs. Presen...

Parinita Agarwal, Pradeep K. Agarwal, Suresh Nair… in Molecular Genetics and Genomics (2007)

Article

A novel isoform of ATPase c subunit from pearl millet that is differentially regulated in response to salinity and calcium

Vacuolar ATPases help in maintaining the pH of the vacuoles and thereby play a crucial role in the functioning of vacuolar sodium-proton antiporter. Though the various subunits that make V₁ and V₀ sector have bee...

Wricha Tyagi, SnehLata Singla-Pareek, Suresh Nair, M. K. Reddy… in Plant Cell Reports (2006)

Chapter

Grape (Vitis vinifera L.)

D.K. Das, M.K. Reddy, K.C. Upadhyaya… in Protocol for Somatic Embryogenesis in Wood… (2005)

Article

Isolation and characterization of a phospholipase C delta isoform from pea that is regulated by light in a tissue specific manner

Phosphoinositide-specific phospholipases C (PLCs) play an important role in many cellular responses and are involved in the production of secondary messengers. We report the cloning and characterization of a c...

G. Venkataraman, M. Goswami, N. Tuteja, M. K. Reddy… in Molecular Genetics and Genomics (2004)

Article

Molecular characterization of a nuclear topoisomerase II from Nicotiana tabacum that functionally complements a temperature-sensitive topoisomerase II yeast mutant

We have successfully expressed enzymatically active plant topoisomerase II in Escherichia coli for the first time, which has enabled its biochemical characterization. Using a PCR-based strategy, we obtained a ful...

B.N. Singh, Yashwanti Mudgil, S.K. Sopory, M.K. Reddy in Plant Molecular Biology (2003)

Article

Global amplification of cDNA from limiting amounts of tissue

In this study we present an improved polymerase chain reaction (PCR)-based methodology to generate large amounts of high-quality complementary DNA (cDNA) from small amounts of initial total RNA. Global amplifi...

M. K. Reddy, Suresh Nair, S. K. Sopory in Molecular Biotechnology (2002)

Article

Cytoplasmic kinase and phosphatase activities can induce PsaF gene expression in the absence of functional plastids: evidence that phosphorylation/dephosphorylation events are involved in interorganellar crosstalk

PsaF is a nuclear gene for subunit III of the reaction center of photosystem I, and its expression is stimulated by cytokinins and light, when monitored at the mRNA level or at the level of GUS activity directed ...

M. R. Chandok, S. K. Sopory, R. Oelmüller in Molecular Genetics and Genomics (2001)

Article

Novel light-activated protein kinases as key regulators of plant growth and development

Plants have evolved highly sensitive sensory photoreceptor systems to regulate various aspects of their growth and development. Many responses such as seed germination, flowering and dormancy are controlled by...

S. C. Maheshwari, J. P. Khurana, S. K. Sopory in Journal of Biosciences (1999)

Article

Cloning and characterization of a cDNA encoding topoisomerase II in pea and analysis of its expression in relation to cell proliferation

We have isolated and sequenced four overlap** cDNA clones to identify the full-length cDNA for topoisomerase II (PsTopII) from pea. Using degenerate primers, based on the conserved amino acid sequences of ot...

M.K. Reddy, Suresh Nair, Krishna K. Tewari, Yashwanti Mudgil… in Plant Molecular Biology (1999)