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Article
Open AccessThe cryo-EM structure of the acid activatable pore-forming immune effector Macrophage-expressed gene 1
Macrophage-expressed gene 1 (MPEG1/Perforin-2) is a perforin-like protein that functions within the phagolysosome to damage engulfed microbes. MPEG1 is thought to form pores in target membranes, however, its m...
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Article
Open AccessThe first transmembrane region of complement component-9 acts as a brake on its self-assembly
Complement component 9 (C9) functions as the pore-forming component of the Membrane Attack Complex (MAC). During MAC assembly, multiple copies of C9 are sequentially recruited to membrane associated C5b8 to fo...
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Article
Open AccessInhibition of a K9/K36 demethylase by an H3.3 point mutation found in paediatric glioblastoma
An array of oncogenic histone point mutations have been identified across a number of different cancer studies. It has been suggested that some of these mutant histones can exert their effects by inhibiting ep...
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Article
Perforin proteostasis is regulated through its C2 domain: supra-physiological cell death mediated by T431D-perforin
The pore forming, Ca2+-dependent protein, perforin, is essential for the function of cytotoxic lymphocytes, which are at the frontline of immune defence against pathogens and cancer. Perforin is a glycoprotein st...
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Article
Open AccessStructure of the poly-C9 component of the complement membrane attack complex
The membrane attack complex (MAC)/perforin-like protein complement component 9 (C9) is the major component of the MAC, a multi-protein complex that forms pores in the membrane of target pathogens. In contrast ...
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Article
Open AccessMaspin is not required for embryonic development or tumour suppression
Maspin (SERPINB5) is accepted as an important tumour suppressor lost in many cancers. Consistent with a critical role in development or differentiation maspin knockout mice die during early embryogenesis, yet ...
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Article
The structural basis for membrane binding and pore formation by lymphocyte perforin
The pore-forming immunity protein perforin, which is essential for the elimination of virally infected and cancerous cells, is released by natural killer and cytotoxic T cells. The structure of a perforin mono...
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Article
GABA production by glutamic acid decarboxylase is regulated by a dynamic catalytic loop
Gamma-aminobutyric acid (GABA) is synthesized by two isoforms of the pyridoxal 5′-phosphate–dependent enzyme glutamic acid decarboxylase (GAD65 and GAD67). GAD67 is constitutively active and is responsible for...
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Article
Molecular genetic analysis of the central hydrophobic domain of subunit 8 of yeast mitochondrial ATP synthase
Subunit 8 (Y8) of yeast mitochondrial ATP synthase (mtATPase) is a hydrophobic component of the membrane Fo sector. Encoded by the mitochondrial aap1 gene, Y8 is a 48-amino-acid polypeptide having a central hydr...
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Protocol
Import into isolated yeast mitochondria of radiolabeled proteins synthesized in vitro
Much of our current understanding of the mechanisms of cellular protein targeting and membrane translocation of proteins derives from studies on mitochondria. The mitochondrial import process has been studied ...
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Chapter
Molecular Biology and Assembly of Yeast Mitochondrial ATP Synthase
This work concerns the structure, function and assembly of the non-F1 proteins of the mitochondrial ATP synthase complex (mtATPase). A variety of in vitro and in vivo approaches based on molecular genetic manipul...
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Chapter
Subunit 8 of Yeast Mitochondrial ATP Synthase: Biochemical Genetics and Membrane Assembly
Subunit 8 is a small integral membrane protein of the proton-translocating F0 sector of the mitochondrial ATP synthase complex. We here review our current understanding of the structure, expression and membrane i...
