38 Result(s)
within
Richard D. Palmiter
Science, Humanities and Social Sciences, multidisciplinary
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Article
What regulates Lactose Content in Milk?
The lactose content of the milk of many species varies inversely with the protein and fat content, but the control of its synthesis is not at present understood.
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Article
Transcriptional regulation of mouse liver metallothionein-I gene by glucocorticoids
Glucocorticoid hormones play an important part in the regulation of many essential metabolic processes1. These include the synthesis of stress-related ‘acute-phase’ proteins2 and the timing of developmental event...
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Article
Structure of mouse metallothionein-I gene and its mRNA
Metallothioneins are small cysteine-rich proteins that bind heavy metals such as zinc, cadmium, copper and mercury1,2. Recent interest in these proteins has focused on the part they play in zinc metabolism and he...
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Article
Regulation of metallothionein–thymidine kinase fusion plasmids injected into mouse eggs
A plasmid was constructed with the promoter/regulatory region of the mouse metallothionein-I gene fused to the structural gene of herpesvirus thymidine kinase. When mouse eggs were microinjected with this plas...
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Article
Dramatic growth of mice that develop from eggs microinjected with metallothionein–growth hormone fusion genes
A DNA fragment containing the promoter of the mouse metallothionein-I gene fused to the structural gene of rat growth hormone was microinjected into the pronuclei of fertilized mouse eggs. Of 21 mice that deve...
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Article
Partial correction of murine hereditary growth disorder by germ-line incorporation of a new gene
The dwarf little (lit) mouse is a model for the human hereditary disorder, isolated growth hormone (GH) deficiency type I1. In these animals, dwarfism results from an autosomal recessively inherited gene mutation...
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Article
Specific expression of an elastase–human growth hormone fusion gene in pancreatic acinar cells of transgenic mice
Transfection of genes into tissue culture cell lines has demonstrated that relatively short DNA sequences can allow expression of inmiunoglobulin1–3, insulin and chymotrypsin4 genes in their appropriate cell type...
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Article
Production of transgenic rabbits, sheep and pigs by microinjection
Direct microinjection has been used to introduce foreign DNA into a number of terminally differentiated cell types as well as embryos of several species including sea urchin1, Candida elegans2, Xenopus3, Drosophi...
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Article
SV40 enhancer and large-T antigen are instrumental in development of choroid plexus tumours in transgenic mice
We have shown recently that choroid plexus tumours frequently develop in transgenic mice which have developed from fertilized eggs injected with DNA molecules containing both simian virus 40 (SV40) early-regio...
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Article
Peripheral neuropathies, hepatocellular carcinomas and islet cell adenomas in transgenic mice
The ability to introduce foreign DNA into the genome of mice offers unique opportunities to produce new models of disease processes. Recent experiments have shown that integration and expression of simian viru...
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Article
Identification of multiple metal regulatory elements in mouse metallothionein-I promoter by assaying synthetic sequences
Metallothionein genes are transcriptionally regulated by a number of inducers including heavy metals. Previous mutational analyses of the mouse metallothionein-I gene (mMTI) promoter have delineated a heavy-metal...
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Chapter
Analysis of the Detoxification of Heavy Metal Ions by Mouse Metallothionein
A mouse hepatoma cell line selected for resistance to Cd (Cdr 80 hepa) was compared to the unselected parental hepatoma cell line (Cds hepa), in terms of its sensitivity to a variety of metal io...
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Chapter
Molecular Biology of Metallothionein Gene Expression
During the seven years since the 1st International Meeting on Metallothionein, the metallothionein (MT) genes have been isolated from a number of organisms and the molecular analysis of their expression has begun...
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Article
Antigen presenting function of class II MHC expressing pancreatic beta cells
Class II major histocompatibility complex (MHC) gene expression in the mouse is generally limited to thymic epithelium and bone marrow-derived cells such as B lymphocytes and cells of the macrophage/dendritic ...
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Article
Genetically haploid spermatids are phenotypically diploid
Because chromosomal homologues segregate from one another during meiosis, spermatids are genetically different. Post-meiotic gene expression could lead to gametic differences, some of which might lead to prefe...
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Article
Transgenic mice overexpressing the mouse homoeobox-containing gene Hox-1.4 exhibit abnormal gut development
The mouse homoeobox-containing genes exhibit temporally and spatially specific patterns of expression in embryonic and adult tissues and are thought to be important in regulation of development and cellular di...
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Article
Abnormal sexual development in transgenic mice chronically expressing Müllerian inhibiting substance
MÜLLERIAN inhibiting substance (MIS), also known as anti-Müllerian hormone, is a glycoprotein1–4 normally secreted by the Sertoli cells of the fetal and adult testis5,6 and by granulosa cells of the postnatal ova...
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Article
Targeted disruption of the tyrosine hydroxylase gene reveals that catecholamines are required for mouse fetal development
TYROSINE hydroxylase catalyses the initial, rate-limiting step in the catecholamine biosynthetic pathway. Catecholamines, which include dopamine, noradrenaline, and adrenaline, are important neurotransmitters ...
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Article
Noradrenaline is essential for mouse fetal development
CATECHOLAMINES such as noradrenaline and adrenaline have been implicated in numerous physiological processes1–4 but, although catecholamine synthesis begins at mid-gestation5, previous studies have provided littl...
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Article
Sensitivity to leptin and susceptibility to seizures of mice lacking neuropeptide Y
NEUROPEPTIDE Y (NPY), a 36-amino-acid transmitter distributed throughout the nervous system1,2, is thought to function as a central stimulator of feeding behaviour1–4. NPY has also been implicated in the modulat...
