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Article
Improving Rice Ability to Degrade Different Polycyclic Aromatic Hydrocarbons Through Multigene Transformation of a Hybrid Dioxygenase System
The widespread presence and potential harm conferred by polycyclic aromatic hydrocarbons (PAHs) to various organisms have raised increased research interest on these pollutants. Plants lack of efficient PAH de...
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Article
A Rice OsAP23, Functioning as an AP2/ERF Transcription Factor, Reduces Salt Tolerance in Transgenic Arabidopsis
The AP2/ERF transcription factors of higher plants regulate transcriptional networks that control development and responses to biotic and abiotic stresses. In this study, the rice OsAP23 gene, which encodes an AP...
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Protocol
Directed In Vitro Evolution of Reporter Genes Based on Semi-Rational Design and High-Throughput Screening
Marker genes, such as gusA, lacZ, and gfp, have been applied comprehensively in biological studies. Directed in vitro evolution provides a powerful tool for modifying genes and for studying gene structure, expres...