Abstract
Although glutathione S-transferase (GST, EC 2.5.1.18) is thought to play important roles in abiotic stress, limited information is available regarding the function of its gene in grapes. In this study, a GST gene from grape, VvGSTF13, was cloned and functionally characterized. Transgenic Arabidopsis plants containing this gene were normal in terms of growth and maturity compared with control plants but had enhanced resistance to salt, drought, and methyl viologen stress. The increased tolerance of the transgenic plants correlated with changes in activities of antioxidative enzymes. Our results indicate that the gene from grape plays a positive role in improving tolerance to salinity, drought, and methyl viologen stresses in Arabidopsis.
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Abbreviations
- CDNB:
-
1-chloro-2,4-dinitrobenzene
- CP:
-
control plants
- GST:
-
glutathione S-transferases
- MV:
-
methyl viologen
- OE:
-
GST-overexpressing lines of Arabidopsis
- ROS:
-
reactive oxygen species
- T:
-
transgenic plant generation
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Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM17-257, March 19, 2018.
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Xu, J., Zheng, AQ., **ng, XJ. et al. Transgenic Arabidopsis Plants Expressing Grape Glutathione S-Transferase Gene (VvGSTF13) Show Enhanced Tolerance to Abiotic Stress. Biochemistry Moscow 83, 755–765 (2018). https://doi.org/10.1134/S0006297918060135
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DOI: https://doi.org/10.1134/S0006297918060135