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Protocol
Probing the Telomere Damage Response
Telomere dysfunctions, rendered through replicative attrition of telomeric DNA or due to the inhibition of shelterin components, are recognized as DNA double-stranded breaks (DSBs) by the DNA damage repair (DD...
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Article
A conserved motif within RAP1 has diversified roles in telomere protection and regulation in different organisms
Telomere protein RAP1 is found in organisms from yeast to mammals, but has different functions. Now the crystal structures of the RAP1 C-terminus (RCT) with its different interacting partners, together with fu...
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Article
TERRA and hnRNPA1 orchestrate an RPA-to-POT1 switch on telomeric single-stranded DNA
Two single-stranded DNA binding proteins, POT1 and RPA, associate with the ends of DNA chromosomes, which are known as telomeres. Binding of RPA to telomeres can activate a DNA damage response, so it was sugge...
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Article
The E3 ubiquitin ligase Rnf8 stabilizes Tpp1 to promote telomere end protection
Rnf8 is an E3 ligase involved in the DNA damage response, adding ubiquitin moieties to histones H2A and H2AX at sites of DNA damage. Now Rnf8 is found to modify shelterin subunit Tpp1, and this is important fo...
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Protocol
Monitoring the DNA Damage Response at Dysfunctional Telomeres
Telomeres are repetitive DNA repeats that cap the ends of all eukaryotic chromosomes. Their proper maintenance is essential for genomic stability and cellular viability. Dysfunctional telomeres could arise thr...
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Article
Open AccessTRF2-RAP1 is required to protect telomeres from engaging in homologous recombination-mediated deletions and fusions
Repressor/activator protein 1 (RAP1) is a highly conserved telomere-interacting protein. Yeast Rap1 protects telomeres from non-homologous end joining (NHEJ), plays important roles in telomere length control a...
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Protocol
Probing the Telomere Damage Response
Telomere dysfunctions, rendered through replicative attrition of telomeric DNA or due to the removal of shelterin components, are recognized as DNA double-stranded breaks (DSBs) by the DNA damage repair (DDR) ...
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Protocol
Cytogenetic Analysis of Telomere Dysfunction
Dysfunctional telomeres arising either through natural attrition due to telomerase deficiency or by the removal of telomere-binding proteins are recognized as double-stranded breaks (DSBs). Repair of DSBs is c...
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Article
Open AccessStructural insights into POT1-TPP1 interaction and POT1 C-terminal mutations in human cancer
Mammalian shelterin proteins POT1 and TPP1 form a stable heterodimer that protects chromosome ends and regulates telomerase-mediated telomere extension. However, how POT1 interacts with TPP1 remains unknown. H...
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Article
Structural and functional analyses of the mammalian TIN2-TPP1-TRF2 telomeric complex
Telomeres are nucleoprotein complexes that play essential roles in protecting chromosome ends. Mammalian telomeres consist of repetitive DNA sequences bound by the shelterin complex. In this complex, the POT1-...
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Article
Open AccessMicrocephalin 1/BRIT1-TRF2 interaction promotes telomere replication and repair, linking telomere dysfunction to primary microcephaly
Telomeres protect chromosome ends from inappropriately activating the DNA damage and repair responses. Primary microcephaly is a key clinical feature of several human telomere disorder syndromes, but how micro...
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Article
Open AccessHomology directed telomere clustering, ultrabright telomere formation and nuclear envelope rupture in cells lacking TRF2B and RAP1
Double-strand breaks (DSBs) due to genotoxic stress represent potential threats to genome stability. Dysfunctional telomeres are recognized as DSBs and are repaired by distinct DNA repair mechanisms. RAP1 and ...
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Article
Open AccessAuthor Correction: Homology directed telomere clustering, ultrabright telomere formation and nuclear envelope rupture in cells lacking TRF2B and RAP1
