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Article
Open AccessManufacturing and preclinical toxicity of GLP grade gene deleted attenuated Leishmania donovani parasite vaccine
Centrin1 gene deleted Leishmania donovani parasite (LdCen1−/−) was developed and extensively tested experimentally as an intracellular stage-specific attenuated and immunoprotective live parasite vaccine candidat...
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Article
Open AccessProduction of leishmanin skin test antigen from Leishmania donovani for future reintroduction in the field
The leishmanin skin test was used for almost a century to detect exposure and immunity to Leishmania, the causative agent of leishmaniasis, a major neglected tropical disease. Due to a lack of antigen used for th...
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Chapter
Toward a Safe and Efficacious Pan-Leishmania Vaccine
Leishmaniasis has been designated by the World Health Organization (WHO) as one of the top neglected tropical diseases with significant morbidity and mortality in low- and middle-income countries (LMICs). With...
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Article
Open AccessCentrin-deficient Leishmania mexicana confers protection against Old World visceral leishmaniasis
Leishmaniasis is one of the top neglected tropical diseases with significant morbidity and mortality in low and middle-income countries (LMIC). However, this disease is also spreading in the developed world. C...
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Article
Open AccessCentrin-deficient Leishmania mexicana confers protection against New World cutaneous leishmaniasis
Leishmaniasis is a neglected protozoan disease affecting over 12 million people globally with no approved vaccines for human use. New World cutaneous leishmaniasis (CL) caused by L. mexicana is characterized by t...
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Article
Open AccessPreclinical validation of a live attenuated dermotropic Leishmania vaccine against vector transmitted fatal visceral leishmaniasis
Visceral Leishmaniasis (VL), a potentially fatal disease is caused by Leishmania donovani parasites with no vaccine available. Here we produced a dermotropic live attenuated centrin gene deleted Leishmania major ...
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Article
Open AccessA second generation leishmanization vaccine with a markerless attenuated Leishmania major strain using CRISPR gene editing
Leishmaniasis is a neglected tropical disease caused by Leishmania protozoa transmitted by infected sand flies. Vaccination through leishmanization with live Leishmania major has been used successfully but is no ...
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Article
Open AccessRole of Mast Cells in clearance of Leishmania through extracellular trap formation
Mast Cells (MCs) are one of the first immune cells encountered by invading pathogens. Their presence in large numbers in the superficial dermis, where Leishmania is encountered, suggests that they may play a crit...
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Article
Open AccessGene deleted live attenuated Leishmania vaccine candidates against visceral leishmaniasis elicit pro-inflammatory cytokines response in human PBMCs
Currently no effective vaccine is available for human visceral leishmaniasis(VL) caused by Leishmania donovani. Previously, we showed that centrin1 and p27gene deleted live attenuated Leishmania parasites (LdCen1
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Article
Open AccessApplication of rapid in vitro co-culture system of macrophages and T-cell subsets to assess the immunogenicity of dogs vaccinated with live attenuated Leishmania donovani centrin deleted parasites (LdCen−/−)
Live attenuated Leishmania donovani parasites as LdCen−/− were shown to confer protective immunity against Leishmania infection in mice, hamsters, and dogs. Strong immunogenicity in dogs vaccinated with LdCen−/− ...
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Protocol
Methods to Evaluate the Preclinical Safety and Immunogenicity of Genetically Modified Live-Attenuated Leishmania Parasite Vaccines
Live-attenuated parasite vaccines are being explored as potential vaccine candidates since other approaches of vaccination have not produced an effective vaccine so far. In order for live-attenuated parasite v...
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Chapter
Current Status and Future Challenges for the Development of Genetically Altered Live Attenuated Leishmania Vaccines
Leishmaniasis is a protozoan parasitic disease endemic to the tropical and subtropical regions of the world, with three major clinical forms, self-healing cutaneous leishmaniasis, mucocutaneous leishmaniasis, ...
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Article
Correction: Corrigendum: Cholesterol depletion associated with Leishmania major infection alters macrophage CD40 signalosome composition and effector function
Nat. Immunol. 10, 273–280 (2009); published online 8 February 2009; corrected after print 18 May 2009 In the version of this article initially published, the GenBank accession number for the Indian human immun...
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Article
Cholesterol depletion associated with Leishmania major infection alters macrophage CD40 signalosome composition and effector function
CD40 signals induce the production of interleukin 12 and interleukin 10 in uninfected and Leishmania major–infected macrophages, respectively. Saha and colleagues suggest that L. major–induced cholesterol depleti...
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Article
An unusual pro-inflammatory role of interleukin-10 induced by arabinosylated lipoarabinomannan in murine peritoneal macrophages
Various species of Mycobacteria produce a major cell wall-associated lipoglycan, called Lipoarabinomannan (LAM), which is involved in the virulence of Mycobacterial species. In this study, we tried to establish t...
