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Article
Open AccessGeneration of light-producing somatic-transgenic mice using adeno-associated virus vectors
We have previously designed a library of lentiviral vectors to generate somatic-transgenic rodents to monitor signalling pathways in diseased organs using whole-body bioluminescence imaging, in conscious, free...
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Protocol
Continual Conscious Bioluminescent Imaging in Freely Moving Mice
In vivo bioluminescent imaging allows the detection of reporter gene expression in rodents in real time. Here we describe a novel technology whereby we can generate somatotransgenic rodents with the use of a v...
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Article
Open AccessProduction of lentiviral vectors using novel, enzymatically produced, linear DNA
The manufacture of large quantities of high-quality DNA is a major bottleneck in the production of viral vectors for gene therapy. Touchlight Genetics has developed a proprietary abiological technology that ad...
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Article
Open AccessArgininosuccinic aciduria fosters neuronal nitrosative stress reversed by Asl gene transfer
Argininosuccinate lyase (ASL) belongs to the hepatic urea cycle detoxifying ammonia, and the citrulline-nitric oxide (NO) cycle producing NO. ASL-deficient patients present argininosuccinic aciduria characteri...
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Article
Open AccessContinual conscious bioluminescent imaging in freely moving somatotransgenic mice
Luciferase bioimaging in living animals is increasingly being applied in many fields of biomedical research. Rodent imaging usually involves anaesthetising the animal during data capture, however, the biologic...
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Article
Open AccessLongitudinal in vivo bioimaging of hepatocyte transcription factor activity following cholestatic liver injury in mice
Molecular mechanisms regulating liver repair following cholestatic injury remain largely unknown. We have combined a mouse model of acute cholestatic liver injury, partial bile duct ligation (pBDL), with a nov...
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Protocol
Bioluminescence Monitoring of Promoter Activity In Vitro and In Vivo
The application of luciferase reporter to provide quantitative outputs for the activation of promoters is a well-established technique in molecular biology. Luciferase catalyzes an enzymatic reaction, which ...
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Article
Open AccessA Broad Overview and Review of CRISPR-Cas Technology and Stem Cells
The pinnacle of four decades of research, induced pluripotent stem cells (iPSCs), and genome editing with the advent of clustered, regularly interspaced, short palindromic repeats (CRISPR) now promise to take ...
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Article
Open AccessIn vivo bioimaging with tissue-specific transcription factor activated luciferase reporters
The application of transcription factor activated luciferase reporter cassettes in vitro is widespread but potential for in vivo application has not yet been realized. Bioluminescence imaging enables non-invasive...
