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Article
In vitro shortening of generation time in Arabidopsis thaliana
Shortening of generations is very important for gene validation and a rapid exploitation of genetic novelties. Even if the life cycle of Arabidopsis thaliana is theoretically short, shortening it further would be...
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Article
Effect of ethrel, chlormequat chloride and paclobutrazol on growth and pyrethrins accumulation in Chrysanthemum cinerariaefolium Vis.
Pyrethrins and flower yield of pyrethrum (Chrysanthemum cinerariaefolium Viz.) plants were determined after application of ethrel, chlormequat chloride and paclobutrazol. Ethrel at 50, 100...
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Article
In vitro morphogenesis from cotyledon and epicotyl explants and flow cytometry distinction between landraces of Bambara groundnut [Vigna subterranea (L.) Verdc], an under-utilised grain legume
The morphogenetic competence of Bambara groundnut was assessed for different landraces, explant sources and media compositions. With cotyledon explants, the best callusing occurred on a medium containing 3 mg/...
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Article
Barley anther culture: effects of annual cycle and spike position on microspore embryogenesis and albinism
The effect of donor plants annual cycle and anther/spike position on the production of microspore-derived plants and albinism were studied. We used the winter cv. Igri and the spring cv. Cork, known to respond...
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Article
Transformation of pollen embryo-derived explants by Agrobacterium tumefaciens in Hyoscyamus niger
Leaf, root, stem, petiole, hypocotyl, and zygotic embryo explants, as well as pollen embryoids, and redifferentiated tissues from pollen embryoid-derived plantlets of Hyoscyamus niger L. (black henbane) were inoc...
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Article
Genetic diversity analysis in Boerhavia diffusa L. of different geographic locations in India using RAPD markers
Boerhavia diffusa is extensively used in herbal medicines as well as in the Ayurvedic system, because it contains a set of clinically important compounds. In the present study, the genetic variability in Boerhav...
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Article
Efficient in vitro direct shoot organogenesis and regeneration of fertile plants from embryo explants of Bambara groundnut (Vigna subterranea L. Verdc.)
An efficient protocol has been developed for direct shoot organogenesis from embryo axes derived from mature seeds of two different landraces of Bambara groundnut. Multiple shoots were initiated on several med...
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Article
Differential morphogenetic responses, ginsenoside metabolism and RAPD patterns of three Panax species
Genetic and metabolomic demarcations between two Indian and one American congeners of Genus Panax have been discerned. Genomic DNA was isolated from the root derived callus cultures of these species and amplified...
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Article
Regulation of essential oil production in plants
This review provides a summary of the physiological dynamics andregulation of essential oil production, from the literature and availableinformation on diverse volatile oil crops. Essential oil production is h...
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Article
Isolation of genomic DNA from defatted oil seed residue of opium poppy (Papaver sominiferum)
A simple protocol for obtaining pure, restrictable and amplifiable megabase genomic DNA from oil free seed residue ofPapaver sominiferum, an important oil seed and morphanian alkaloid yielding plant, has been dev...
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Article
Plastid differentiation during androgenesis in albino and non-albino producing cultivars of barley (Hordeum vulgare L.)
In order to better understand androgenic albinism in barley, we compared plastid differentiation during anther culture in two cultivars, an albino (spring cultivar Cork) and a non-albino (winter cultivar Igri)...
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Article
Identification of Drechslera Graminea Isolates by Cultural Characters and Rapd Analysis
Isolates of Drechslera graminea, the causal agent of barley leaf stripe, were collected from different regions of Syria and analyzed for differences in cultural morphology, growth rate, colony colour, and randoml...
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Article
Essential Oil Gland Number and Ultrastructure During Mentha arvensis Leaf Ontogeny
Alterations in essential oil gland number, distribution and fine structure, and the oil content in the leaf of Mentha arvensis L. were examined during its growth and senescence. Accumulation of essential oil o...
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Article
Effect of different photoperiodic regimes on growth, flowering and essential oil in Mentha species
Three major cultivated Mentha spp. (M. arvensis, M. citrata and M. cardiaca) were grown under short-days, normal-days or long-days for 60 cycles. Subsequent to photoperiodic treatment, the plants were assessed...
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Article
Differential development of plastids during microspore embryogenesis in barley
In order to understand and limit albino plantlet formation during pollen embryogenesis in barley (Hordeum vulgare L. cv. Igri), plastid feature was followed during pollen embryogenesis under two anther culture co...
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Article
Isolation of Genomic DNA from the Antimalarial Plant Artemisia annua
A suitable protocol is described to obtain high molecular weight, restrictable, and amplifiable genomic DNA from the antimalarial plant Artemisia annua. The method is a CTAB procedure that includes a rapid mic...
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Article
Effect of ploidy and homozygosity on transgene expression in primary tobacco transformants and their androgenetic progenies
Expression of a transgene is rarely analysed in the androgenetic progenies of the transgenic plants. Here, we report differential transgene expression in androgenetic haploid and doubled haploid (DH) tobacco ...
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Article
Inheritance of flower and pod colour in cowpea (Vigna unguiculata L. Walp.)
Inheritance of flower colour and pod colour in cowpea (Vigna unguiculata L. Walp.) has followed a qualitative pattern. Purple flower colour is dominant over white flower colour, whereas black pod colour is par...
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Article
Flow cytometric analyses in embryogenic and non-embryogenic callus lines of Cyclamen persicum Mill.: relation between ploidy level and competence for somatic embryogenesis
Embryogenic and non-embryogenic callus lines derived from the same diploid Cyclamen persicum genotype (`Purple Flamed') were analyzed by flow cytometry and compared to the initial plant material. The DNA content...
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Article
Factors influencing secondary somatic embryogenesis inMalus x domestica Borkh. (cv ‘Gloster 69’)
A procedure for regeneration of apple plants through secondary somatic embryogenesis (SSE) was developed in apple ‘Gloster 69’. Primary somatic embryos were produced from cotyledon-derived cultures of immature...
