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Article
National Scientific Medical Meeting 1994 Abstracts
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Article
Identification of the cytokinins in red pine seedlings
Zeatin-9-riboside was identified in shoots and roots of Pinus resinosa by GC-MS analysis of its permethyl derivative. Based on their chromatographic properties on Sephadex LH-20 and C18 HPLC, and their susceptibi...
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Article
Effects of abscisic acid analogues on abscisic acid-induced gene expression in barley aleurone protoplasts: Relationship between structure and function of the abscisic acid molecule
The plant hormone abscisic acid (ABA) mediates gene expression in barley aleurone protoplasts. In order to elucidate the essential functional groups of the ABA molecule, the specificity of a number of ABA anal...
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Article
The role of cis-carotenoids in abscisic acid biosynthesis
Evidence has been obtained which is consistent with 9′-cis-neoxanthin being a major precursor of abscisic acid (ABA) in higher plants. A mild, rapid procedure was developed for the extraction and analysis of caro...
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Chapter and Conference Paper
Cytokinin Oxidase and the Degradative Metabolism of Cytokinins
Over the last 16 years considerable knowledge has accumulated about the metabolism of naturally occurring and synthetic cytokinins. In the case of naturally occurring cytokinins, many of the metabolites identi...
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Article
The effect of auxin concentration on cytokinin stability and metabolism
The stability of [3H]zeatin riboside supplied to freshly excised tobacco pith explants was found to be inversely related to α-naphthaleneacetic acid concentration in the incubation medium. At higher concentration...
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Article
Apical localization of 1-aminocyclopropane-1-carboxylic acid and its conversion to ethylene in etiolated pea seedlings
The biosynthetic basis for the high rates of ethylene production by the apical region of etiolated pea (Pisum sativum L.) seedlings was investigated. The ethylene precursor 1-aminocyclopropane-1-carboxylic acid (...
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Article
Xanthoxin levels and metabolism in the wild-type and wilty mutants of tomato
Using 13C-labelled internal standards and gas chromatography-mass spectrometry/multiple-ion monitoring the levels of xanthoxin (Xan) and 2-trans-xanthoxin (t-Xan) have been determined in stressed and non-stressed...
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Article
Seed development and vivipary in Zea mays L.
Seed development was investigated in kernels of develo** wild-type and viviparous (vp-1) Zea mays L. Embryos and endosperm of wild-type kernels began to dehydrate at approx. 35 d after pollination (DAP); vivipa...
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Article
Partial isotope fractionation during high-performance liquid chromatography of deuterium-labelled internal standards in plant hormone analysis: A cautionary note
Deuterium-labelled indole-3-acetic acid, abscisic acid and phthalimido-1-aminocyclopropane-1-carboxylic acid were found to separate from the unlabelled compounds on reverse-phase high-performance liquid chroma...
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Article
The carotenoid and abscisic acid content of viviparous kernels and seedlings ofZea mays L.
Carotenoid and abscisic acid (ABA) levels were determined in endosperm, embryos and seedlings of wild-type and viviparous (vp) mutants ofZea mays L. Carotenoid concentrations were determined by absorption spectro...
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Chapter and Conference Paper
Cytokinin Biosynthesis and Metabolism
This article attempts to take a retrospectic look at the progress made in the area of cytokinin biochemistry since the subject was last reviewed at the 11th IPGSA conference at Aberystwyth in 1982. At that mee...
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Article
Cytokinins in transfer RNA of normal and crown-gall tissue of Vinca rosea
cis-Zeatin riboside was identified in transfer-RNA hydrolysates from both normal and crown-gall tissue of Vinca rosea L. The trans-isomer was associated exclusively with the crowngall transfer-RNA. The importanc...
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Article
Determination of the levels of abscisic acid-glucose ester in plants
Abscisic acid-β-D-glucopyranosyl ester (ABAGE) has been measured in several plant species using 2H-labelled ABAGE as an internal standard. The effects of water stress on the levels of ABAGE and its possible physi...
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Article
Cytokinin biosynthesis in crown-gall tissue ofVinca rosea
When care was taken to minimise the effects of phosphatase activity during extraction ofVinca rosea crown-gall tumour tissue, a large proportion of extractable cytolinin activity was present in the nucleotide fra...
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Article
Mass spectrometric measurement of zeatin glycoside levels in Vinca rosea L. crown gall tissue
The range of zeatin glycosides found in crown gall tissue of Vinca rosea L. has been quantified using a mass spectrometric isotope dilution procedure. Problems in the quantitative analysis of cytokinins in plant ...
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Article
Cytokinin metabolism in Phaseolus vulgaris L.
The major cytokinins in stems of decapitated, disbudded bean plants have been identified by enzymic degradation, Sephadex LH20 and reversed phase high performance liquid chromatography, and by combined gas chr...
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Article
Dihydroconiferyl alcohol — A cell division factor from Acer species
A compound that stimulated growth of soybean callus was isolated from spring sap of sycamore (Acer pseudoplatanus L.). Insufficient compound was isolated to permit it to be characterised. A compound with identica...
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Chapter and Conference Paper
Cytokinin Biosynthesis and Metabolism inVinca rosea Crown Gall Tissue
Progress in the understanding of the mechanisms and controls of plant hormone biosynthesis and metabolism has to a large extent been limited by the availability of suitable systems for study. Whilst the ultima...
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Article
Zeatin-9-glucoside, a major endogenous cytokinin of Vinca rosea L. crown gall tissue
Cultured crown gall tissue of Vinca rosea L. was found to contain, in addition to the previously reported cytokinins zeatin, zeatin riboside, and the 0-glucosides of these two compounds, relatively high levels of...
