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Article
Genetic Analysis of the Role of Trichothecene and Fumonisin Mycotoxins in the Virulence of Fusarium
The phytotoxicity of the Fusarium trichothecene and fumonisin mycotoxins has led to speculation that both toxins are involved in plant pathogenesis. This subject has been addressed by examining virulence of trich...
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Article
Variation in Pathogenicity Associated with the Genetic Diversity of Fusarium graminearum
We screened 188 isolates of Fusarium graminearum, which originated from northwest Europe, the USA and Nepal, for genetic diversity using a sequence-characterised amplified region polymorphism (SCAR). On the basis...
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Article
Deoxynivalenol-nonproducing Fusarium graminearum Causes Initial Infection, but does not Cause DiseaseSpread in Wheat Spikes
Fusarium graminearum is a major pathogen that causes fusarium head blight (FHB) in wheat and produces deoxynivalenol (DON) in infected grain. In previous studies, the trichodiene synthase gene (Tri5) in the funga...
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Chapter
Genetic analysis of the role of trichothecene and fumonisin mycotoxins in the virulence of Fusarium
The phytotoxicity of the Fusarium trichothecene and fumonisin mycotoxins has led to speculation that both toxins are involved in plant pathogenesis. This subject has been addressed by examining virulence of trich...
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Article
Genetic and Molecular Aspects of the Biosynthesis of Trichothecenes by Fusarium
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Chapter
Isolation and Purification of Fumonisin B1 and B2 from Rice Culture
Procedures are presented for growing Fusarium moniliforme MRC 826 on rice, separation of fumonisin B1 (FB1) from fumonisin B2 (FB2), purification of FB1 and preliminary procedures for purification of FB2. The myc...
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Article
Microbial transformation of 12-hydroxyoctadecanoic acid to 5-n-hexyl-tetrahydrofuran-2-acetic acid
Stationary-phase cells of a corynebacterium (FUI-2) and a bacillus (NRRL B-14864) isolate, when grown aerobically in 1% YE medium at 25°C, converted 12-hydroxystearic acid to a major compound, 5-n-hexyl-tetrahydr...
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Article
Alanine is a precursor in the biosynthesis of fumonisin B1 byFusarium moniliforme
The biosynthesis of fumonisin B1 byFusarium moniliforme was studied in liquid culture. Stable isotope labeled alanine was added as a precursor to static and shaken cultures ofF. moniliforme. Incorporation of13C a...
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Article
Production of 15-, 16- and 17-hydroxy-9-octadecenoic acids by bioconversion of oleic acid withBacillus pumilus
SeveralBacillus strains were tested for their ability to hydroxylate oleic acid. Two strains—BD-174 and BD-226—converted oleic acid into a trio of hydroxy-octadecenoic acids. Bioconversion in 72–120 hr produced 5...
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Article
Concentrations of fumonisin B1 in feeds associated with animal health problems
Ninety-eight samples of feeds associated with 44 cases of equine leukoencephalomalacia (ELEM) and 83 samples of feed associated with 42 cases of a porcine pulmonary edema syndrome (PPE) were analyzed for fumon...
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Article
A novel compound, 7,10-dihydroxy-8(E)-octadecenoic acid from oleic acid by bioconversion
Sixty-two cultures from the Agricultural Research Service (ARS) Culture Collection and 10 cultures isolated from soil and water samples in Illinois were screened for their ability to convert agricultural oils ...
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Chapter and Conference Paper
An a-Ge:H pin Solar Cell with High Red Efficiency
Solar cells with pin structure were manufactured from pure hydrogenated amorphous germanium in order to obtain a red sensitive sub cell in a tandem stucture. Different deposition conditions were tested. A subs...
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Article
Chemical lonization-mass spectrometry of secondary oxidation products from methyl linoleate and linolenate
Chemical ionization-mass spectrometry (CI-MS) with a direct exposure probe was used to analyze a series of hydroperoxy cyclic peroxides and dihydroperoxides obtained from methyl linoleate and linolenate by eit...
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Article
New long-chain hydroxy acids fromGrevillea decora
In addition to the ω-5 olefinic acids found in otherGrevillea species, about 10% of the acyl groups ofG. decora seed oil contain a hydroxy group and an ω-5 double bond. The chainlengths of these acids are from C2...
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Article
Mass spectrometry of isomeric fatty acid hydroperoxides by chemical ionization via direct exposure probe
Intact isomeric methyl 9- and 13-hydroperoxy linoleates are analyzed by chemical ionization (CI) mass spectrometry using a direct exposure probe. Both isobutane and ammonia CI spectra were obtained. With isobu...
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Article
Linoleate hydroperoxides are cleaved heterolytically into aldehydes by a Lewis acid in aprotic solvent
Treatment of isomeric methyl linoleate hydroperoxides with a Lewis acid, BF3, in anhydrous ether led to a carbon-to-oxygen rearrangement that caused cleavage into shorter-chain aldehydes. Methyl (9Z,11E)-13-hydro...
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Article
Chemical lonization mass spectrometry of fatty acids: The effect of functional groups on the CI spectra1
Chemical ionization (CI) mass spectra of functionally substituted fatty esters are a useful aid in determining molecular weight. Isobutane and ammonia CI mass spectra of various hydroxy, keto, epoxy and hydrop...
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Article
Chemical lonization mass spectrometry of wax esters
Chemical ionization (CI) mass spectra of long-chain saturated, monenoic and dienoic wax ester analogues, using isobutane and methane as reagent gases, are reported and compared. Because less fragmentation occu...
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Article
Isolation and purification of deoxynivalenol and a new trichothecene by high pressure liquid chromatography
Deoxynivalenol (3,7,15-trihydroxy-12,l3-epoxytrichothec-9-ene-8-one) was extracted from corn with methanol/water (80:20, v/v) and purified by liquid:liquid partitioning and by preparative high pressure liquid ...
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Article
High performance liquid chromatography of triglycerides: Controlling selectivity with reverse phase columns
Rapid separations of triglycerides by chain length and degree of unsaturation have been made by high performance liquid chromatography (HPLC) using reverse phase columns. Several different bonded columns were ...
