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Article
Open AccessOptimized Cas9:sgRNA delivery efficiently generates biallelic MSTN knockout sheep without affecting meat quality
CRISPR/Cas9-based genome-editing systems have been used to efficiently engineer livestock species with precise genetic alterations intended for biomedical and agricultural applications. Previously, we have suc...
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Article
Open AccessHighly efficient generation of sheep with a defined FecBB mutation via adenine base editing
Base editing has the potential to improve important economic traits in agriculture and can precisely convert single nucleotides in DNA or RNA sequences into minimal double-strand DNA breaks (DSB). Adenine base...
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Article
Open AccessLow incidence of SNVs and indels in trio genomes of Cas9-mediated multiplex edited sheep
The simplicity of the CRISPR/Cas9 system has enabled its widespread applications in generating animal models, functional genomic screening and in treating genetic and infectious diseases. However, unintended m...
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Article
Open AccessMultiplex gene editing via CRISPR/Cas9 exhibits desirable muscle hypertrophy without detectable off-target effects in sheep
The CRISPR/Cas9 system provides a flexible approach for genome engineering of genetic loci. Here, we successfully achieved precise gene targeting in sheep by co-injecting one-cell-stage embryos with Cas9 mRNA ...
