Cartilage Tissue Engineering
Methods and Protocols
Book
Protocol
Human adult mesenchymal stem cells are present in fat tissue, which can be obtained using surgical procedures such as liposuction. The multilineage capacity of mesenchymal stem cells makes them very valuable f...
Protocol
Mechanical forces, including hydrodynamic shear, hydrostatic pressure, compression, tension, and friction, can have stimulatory effects on cartilage synthesis in tissue engineering systems. Bioreactors capable...
Protocol
Many technologies that underpin tissue engineering as a research field were developed with the aim of producing functional human cartilage in vitro. Much of our practical experience with three-dimensional cult...
Protocol
As the only cell type found in healthy adult cartilage, chondrocytes are the obvious and most direct starting point for cartilage tissue engineering. Human adult, juvenile, neonatal, and fetal chondrocytes hav...
Book
Chapter
Hairy roots are a convenient experimental tool for investigating the interactions between plant cells and metal ions. Hairy roots of species capable of hyperaccumulating Cd and Ni have been applied to investig...
Article
Linum flavum hairy roots were initiated from leaf discs using Agrobacterium rhizogenes strains LBA9402 and TR105 though two other strains, 15834 and A4, were relatively ineffective for induction. Significant var...
Article
Hairy roots and suspended cells of transformedNicotiana tabacum were used to produce full length murine IgG1 monoclonal antibody. The maximum amount of antibody accumulated per g dry weight in the hairy root cult...
Article
The specific growth rate of Atropa belladonna hairy roots measured in terms of root length increased by about 25% to 0.49 d-1 when the inoculum consisted of a root with the primary root tip excised. The biomass d...
Article
Culture characteristics of genetically transformed Mentha citrata shooty teratomas were studied in liquid Murashige and Skoog and Gamborg's B5 media. Based on calculated kinetic and yield parameters, a stoichiome...
Article
Addition of 40 mg l-1 hydroxyurea to Solanium aviculare cultures resulted in complete inhibition of growth and an increase in the levels of steroidal alkaloids in the biomass. These results demonstrate that solas...
Chapter
To be economically feasible, production of most secondary metabolites by plant-cell culture requires high cell densities. In standard reactor configurations such as stirred-tank and airlift vessels, large-scal...
Article
Morphine and codeine accumulation in Papaver somniferum suspension cultures increased markedly after removal of hormones from the medium. Cultures developed hormone self-sufficiency without organogenesis or devel...